CCRL2/CRAM-A/B Antibody (152254) [Allophycocyanin]

Peripheral Blood Mononuclear Cells (PBMCs) cultured with Recombinant Human IL-2 (Catalog # 202-IL) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCRL2/CRAM-A/B APC-conjugated Monoclonal Antibody (Catalog # FAB23501A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

MDA-MB-231 human breast adenocarcinoma cell line was stained with Mouse Anti-Human CCRL2/CRAM-A/B APC-conjugated Monoclonal Antibody (Catalog # FAB23501A, filled histogram) or Mouse IgG2B Allophycocyanin Isotype Control (IC0041A, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: 152254
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

CCRL2/CRAM-A/B Antibody (152254) [Allophycocyanin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CRAM-A
  Met1-Val356 (Val180Met)
  Accession # NP_001124382
• Specificity: Detects human CCRL2/CRAM‑A/B. Stains human CCRL2-transfected cells but not irrelevant transfectants. Detects both the CRAM-A and CRAM-B isoforms.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCRL2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCRL2/CRAM-A/B Antibody (152254) [Allophycocyanin]

• ACKR5
• C-C chemokine receptor-like 2
• CCR11
• CCR6
• CCRL2
• CCRL2/CRAM-A/B
• chemokine (C-C motif) receptor-like 2
• Chemokine receptor CCR11
• Chemokine receptor X
• CKRX
• CKRXMGC116710
• CRAM
• CRAMA
• CRAM-A
• CRAMB
• CRAM-B
• FLJ55815
• HCR
• HCRMGC34104
• Putative MCP-1 chemokine receptor

Background

CCRL2, also known as CCR11, CRAM-A, CRAM-B, CKRX, and HCR, is a seven-transmembrane G-protein linked receptor that shares homology with other human chemokine receptors. The isoforms CRAM-A and CRAM-B differ at their N-termini by the insertion of an additional 12 amino acids in CRAM-A. CCRL2/CRAM‑A/B is expressed at varying levels on a variety of peripheral blood cells including monocytes, neutrophils, B cells, and T cells (1, 2). CCL19 has been identified as a ligand for CRAM-B (4). Human and mouse CRAM share approximately 47% amino acid sequence identity.

1. Fan, P. et al. (1998) Biochem. Biophys. Res. Commun. 243:264.
2. Migeotte, I. et al. (2002) Eur. J. Immunol. 32:494.
3. Hartmann, T.N. et al. (2008) Immunology 125:252.
4. Leick, M. et al. (2010) Immunology 129:536.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCRL2/CRAM-A/B Antibody (FAB23501A)(2)

Publications using FAB23501A

• Paola Vinci Mesenchymal stromal cell-secreted chemerin is a novel immunomodulatory molecule driving the migration of ChemR23-expressing cells Cytotherapy, 2016-12-06;0(0):. 2016-12-06 [PMID: 27939374] (Flow Cytometry, Human)
• Vida Farsam, Abhijit Basu, Martina Gatzka, Nicolai Treiber, Lars A. Schneider, Medhanie A. Mulaw et al. Senescent fibroblast-derived Chemerin promotes squamous cell carcinoma migration Oncotarget 12/13/2016 [PMID: 27907906]

Bioinformatics

• Gene Symbol: CCRL2
• Uniprot:
  • NP_001124382()