CCR5 Antibody (T21/8) - BSA Free

Flow Cytometry: CCR5 Antibody (T21/8) [NBP1-43335] - Analysis using the PE conjugate of NBP1-43335. Staining of normal human peripheral blood cells with Anti-Human CD3 APC and Mouse IgG1 kappa Isotype Control PE (left) or Anti-Human CD195 (CCR5) PE (right). Cells in the lymphocyte gate were used for analysis.

Flow Cytometry: CCR5 Antibody (T21/8) [NBP1-43335] - Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human CD195 (CCR5) Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE. Cells in the lymphocyte gate were used for analysis.

Flow Cytometry: CCR5 Antibody (T21/8) [NBP1-43335] - Staining of normal human peripheral blood cells with Anti-Human CD3 FITC and 0.25 ug of Mouse IgG1 kappa Isotype Control BIOTIN (left) or 0.25 ug of Anti-Human CD195 (CCR5) BIOTIN (right) followed by Streptavidin PE. Cells in the lymphocyte gate were used

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, IP
• Clone: T21/8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CCR5 Antibody (T21/8) - BSA Free Summary

• Description: Novus Biologicals Mouse CCR5 Antibody (T21/8) - BSA Free (NBP1-43335) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody was CCR5.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry </= 0.5 ug/10^6 cells in 100 ul
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CCR5 Antibody (T21/8) - BSA Free

• C-C CKR-5
• C-C motif chemokine receptor 5 A159A
• CCCKR5
• CC-CKR-5FLJ78003
• CCR5
• CCR-5
• CD195 antigen
• CD195
• chemokine (C-C motif) receptor 5
• chemokine receptor CCR5
• chemr13
• CKR5
• CKR-5
• HIV-1 fusion coreceptor
• IDDM22CMKBR5C-C chemokine receptor type 5

Background

The T21/8 monoclonal antibody reacts with the amino terminus of human CCR5 (CD195), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1alpha, MIP-1beta and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120. Binding of the T21/8 monoclonal antibody displays donor variability and may be affected by sub-optimal staining conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR5 Antibody (NBP1-43335)(1)

Publication using NBP1-43335

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: CCR5
• Entrez:
  • 1234(Human)
• Uniprot:
  • P51681()