CCR5 Antibody (45531) [Phycoerythrin]

NS0 mouse myeloma cell line transfected with human CCR5 was stained with Mouse Anti-Human CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB182P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 45531
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CCR5 Antibody (45531) [Phycoerythrin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CCR5
  Met1-Leu352
  Accession # P51681
• Specificity: Detects human CCR5.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR5
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR5 Antibody (45531) [Phycoerythrin]

• C-C CKR-5
• C-C motif chemokine receptor 5 A159A
• CCCKR5
• CC-CKR-5FLJ78003
• CCR5
• CCR-5
• CD195 antigen
• CD195
• chemokine (C-C motif) receptor 5
• chemokine receptor CCR5
• chemr13
• CKR5
• CKR-5
• HIV-1 fusion coreceptor
• IDDM22CMKBR5C-C chemokine receptor type 5

Background

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha , MIP-1 beta , MCP-2, and RANTES. It also functions as a coreceptor for macrophage tropic HIV-1 infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR5 Antibody (FAB182P)(9)

Publications using FAB182P

• Harms, M;Smith, N;Han, M;Gro beta , R;von Maltitz, P;Stürzel, C;Ruiz-Blanco, YB;Almeida-Hernández, Y;Rodriguez-Alfonso, A;Cathelin, D;Caspar, B;Tahar, B;Sayettat, S;Bekaddour, N;Vanshylla, K;Kleipass, F;Wiese, S;Ständker, L;Klein, F;Lagane, B;Boonen, A;Schols, D;Benichou, S;Sanchez-Garcia, E;Herbeuval, JP;Münch, J; Spermine and spermidine bind CXCR4 and inhibit CXCR4- but not CCR5-tropic HIV-1 infection Science advances 2023-07-07 [PMID: 37406129] (Bioassay, Flow Cytometry, Human)
• Martin J. Scott, Amanda Jowett, Martin Orecchia, Peter Ertl, Larissa Ouro-Gnao, Julia Ticehurst et al. Rapid identification of highly potent human anti-GPCR antagonist monoclonal antibodies mAbs 2020-04-29 [PMID: 32343620]
• Morandi F, Prigione I, Airoldi I Human TCR gamma delta+ T cells represent a novel target for IL-27 activity. Eur. J. Immunol., 2012-06-01;42(6):1547-52. 2012-06-01 [PMID: 22678908] (Flow Cytometry, Human)
• Badr G, Lefevre EA, Mohany M Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis. PLoS ONE, 2011-09-01;6(9):e23741. 2011-09-01 [PMID: 21912642] (Flow Cytometry, Human)
• Julie Catusse, Marion Leick, Mareike Groch, David J Clark, Maike V Buchner, Katja Zirlik et al. Role of the atypical chemoattractant receptor CRAM in regulating CCL19 induced CCR7 responses in B-cell chronic lymphocytic leukemia Molecular Cancer 2010-12-01 [PMID: 21092185]
• Brucklacher-Waldert V, Steinbach K, Lioznov M, Kolster M, Holscher C, Tolosa E Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression. J. Immunol., 2009-11-01;183(9):5494-501. 2009-11-01 [PMID: 19843935] (Flow Cytometry, Human)
• Ahern D, Lloyd CM, Robinson DS Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors. Allergy, 2009-02-07;64(8):1121-9. 2009-02-07 [PMID: 19208087] (Flow Cytometry, Human)
• Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008-12-01;181(11):8053-67. 2008-12-01 [PMID: 19017998] (Flow Cytometry, Human)
• Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy. Int. J. Cancer, 2006-05-01;118(9):2147-57. 2006-05-01 [PMID: 16331601] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCR5
• Uniprot:
  • P51681()