CCR5 Antibody (45529)

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 45529
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR5 Antibody (45529) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CCR5
  Met1-Leu352
  Accession # P51681
• Specificity: Detects human CCR5. Specifically stains human CCR5 transfectants but not the parental cell lines. It does not cross-react with human CCR1, CCR2, or CCR3. Can be used to detect CCR5 present on stimulated human PBMCs. For additional information regarding epitope specificity for this antibody and other R&D Systems anti-human CCR5 antibodies, see Lee, B. et al. (1999) J. Biol. Chem. 274:9617.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR5
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR5 Antibody (45529)

• C-C CKR-5
• C-C motif chemokine receptor 5 A159A
• CCCKR5
• CC-CKR-5FLJ78003
• CCR5
• CCR-5
• CD195 antigen
• CD195
• chemokine (C-C motif) receptor 5
• chemokine receptor CCR5
• CHEMR13
• CKR5
• CKR-5
• HIV-1 fusion coreceptor
• IDDM22CMKBR5C-C chemokine receptor type 5

Background

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha , MIP-1 beta , MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR5 Antibody (MAB184)(6)

Publications using MAB184

• Shengli Song, Miriam Manook, Jean Kwun, Annette M. Jackson, Stuart J. Knechtle, Garnett Kelsoe A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines Communications Biology 11/25/2021 [PMID: 34824350]
• Structural Insights into the Inhibitory Mechanism of an Antibody against B7-H6, a Stress-Induced Cellular Ligand for the Natural Killer Cell Receptor NKp30 J Mol Biol, 2016-09-20;0(0):. 2016-09-20 [PMID: 27663271] (Flow Cytometry, Mouse)
• Alonzo , Francis, Kozhaya , Lina, Rawlings , Stephen, Reyes-Robles , Tamara, DuMont , Ashley L, Myszka , David G, Landau , Nathanie, Unutmaz , Derya, Torres , Victor J CCR5 is a receptor for Staphylococcus aureus leukotoxin ED. Nature, 2012-12-12;493(7430):51-5. 2012-12-12 [PMID: 23235831] (Bioassay, Human)
• Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6. Int. Immunol., 2009-07-10;21(8):991-1001. 2009-07-10 [PMID: 19592420] (Flow Cytometry, Mouse)
• Honczarenko M, Le Y, Glodek AM, Silberstein LE CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor. Blood, 2002-10-01;100(7):2321-9. 2002-10-01 [PMID: 12239139] (Neutralization, Human)
• Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function. J. Biol. Chem., 1999-04-02;274(14):9617-26. 1999-04-02 [PMID: 10092648] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCR5
• Uniprot:
  • P51681()