CCR2 Antibody (E68)

Product Discontinued

CCR2 Antibody (E68) Summary

• Immunogen: A synthetic peptide corresponding to the N-terminal residues of the human C-C chemokine receptor type 2 was used as immunogen.
• Specificity: The antibody does not cross-react with other CKR family members.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CCR2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:50
  • Western Blot 1:100-1:2000
• Application Notes: In Western blot this antibody detects a band at approximately 42-52 kDa. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
• Control:

  CCR2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 59% PBS, 0.05% BSA and 40% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for CCR2 Antibody (E68)

• C-C chemokine receptor type 2
• C-C CKR-2
• CC-CKR-2
• CC-CKR-2CKR2B
• CCR2
• CCR-2
• CCR2A
• CCR2B
• CD192 antigen
• CD192
• chemokine (C-C motif) receptor 2
• CKR2
• CKR2A
• CMKBR2
• CMKBR2MGC111760
• FLJ78302
• MCP-1 receptor
• MCP-1-R
• MCP-1-RMGC103828
• MGC168006
• Monocyte chemoattractant protein 1 receptor
• monocyte chemotactic protein 1 receptor

Background

CCR2, or C C chemokine receptor type 2, is a receptor for several monocyte chemo attractant proteins (MCP1, MCP3, MCP4) which specifically mediate monocyte chemotaxis. CCR2 transduces such signals by increasing the intracellular level of calcium ions. For example, MCP1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. CCR2 is also an alternative coreceptor with CD4 for HIV1 infection. CCR2 has two isoforms and has been reported to be expressed in a wide variety of tissues, including blood, brain, heart, kidney, liver, lung, ovary, pancreas, spinal cord, spleen and thymus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR2 Antibody (NB110-55674)(24)

Publications using NB110-55674

• Kozireva S, Rudevica Z, Baryshev M et al. Upregulation of the Chemokine Receptor CCR2B in Epstein-Barr Virus-Positive Burkitt Lymphoma Cell Lines with the Latency III Program Viruses 2018-05-03 [PMID: 29751565] (WB)
• Kholodnyuk I, Rudevica Z, Leonciks A et al. Expression of the chemokine receptors CCR1 and CCR2B is up-regulated in peripheral blood B cells upon EBV infection and in established lymphoblastoid cell lines. Virology 2017-09-08 [PMID: 28892735] (Human)
• Liu Y, Gunsten SP, Sultan DH et al. PET-based Imaging of Chemokine Receptor 2 in Experimental and Disease-related Lung Inflammation Radiology 2017-06-01 [PMID: 28045644] (IF/IHC, Mouse)
• Jagadeesh Janjanam, Gadiparthi N. Rao Novel role of cortactin in G proteincoupled receptor agonist-induced nuclear export and degradation of p21Cip1. Scientific Reports 2016-07-01 [PMID: 27363897] (WB, WB, Human)
• Lee YS, Kim SY, Song SJ et al. Crosstalk between CCL7 and CCR3 promotes metastasis of colon cancer cells via ERK-JNK signaling pathways. Oncotarget. 2016-06-14 [PMID: 27167205] (WB, Human)
• Jagadeesh Janjanam, Giri Kumar Chandaka, Sivareddy Kotla et al. PLC3 mediates cortactin interaction with WAVE2 in MCP1-induced actin polymerization and cell migration. The American Society of Cell Biology 2015-10-13 [PMID: 26490115] (IP, WB, Human)
• He Shizhi, He Shuangba, Chen Chun-Hao et al. The chemokine (CCL2-CCR2) signaling axis mediates perineural invasion. Molecular Cancer Research : Mcr 2015-01-01 [PMID: 25312961] (IF/IHC, Human)
• Nishiwaki S, Nakayama T, Murata M et al. Dexamethasone Palmitate Ameliorates Macrophages-Rich Graft-versus-Host Disease by Inhibiting Macrophage Functions. PLoS ONE. 2014-05-08 [PMID: 24806147] (FLOW, Mouse)
• Stowe AM, Wacker BK, Cravens PD et al. CCL2 upregulation triggers hypoxic preconditioning-induced protection from stroke J Neuroinflammation 2012-02-16 [PMID: 22340958] (WB, Mouse)
• Yao Y, Tsirka SE. The C Terminus of Mouse Monocyte Chemoattractant Protein 1 (MCP1) Mediates MCP1 Dimerization while Blocking Its Chemotactic Potency . J Biol Chem 285 31509-31516. 2010-01-01 [PMID: 20682771]
• Fu ES, Zhang YP, Sagen J et al. Transgenic inhibition of glial NF-kappa B reduces pain behavior and inflammation after peripheral nerve injury. Pain 2010-03-01 [PMID: 20097004] (IF/IHC)
• Ali S, O'Boyle G, Hepplewhite P et al. Therapy with nonglycosaminoglycan-binding mutant CCL7: a novel strategy to limit allograft inflammation. Am J Transplant 2010-01-01 [PMID: 19951286] (IF/IHC)
• Baba T, Nakamoto Y, Mukaida N. Crucial Contribution of Thymic Sirp+ Conventional Dendritic Cells to Central Tolerance against Blood-Borne Antigens in a CCR2-Dependent Manner ,J. Immunol 183 3053 - 3063. 2009-01-01 [PMID: 19675159]
• Tarabra E, Giunti S, Barutta F et al. Effect of the Monocyte Chemoattractant Protein-1/CC Chemokine Receptor 2 System on Nephrin Expression in Streptozotocin-Treated Mice and Human Cultured Podocytes . Diabetes58 2109 - 2118. 2009-01-01 [PMID: 19587356] (IF/IHC)
• Hart KM, Bak SP, Alonso A, Berwin B. Phenotypic and functional delineation of murine CX(3)CR1 monocyte-derived cells in ovarian cancer. Neoplasia 2009-06-01 [PMID: 19484145] (FACS, Mouse)
• Lu P, Li L, Liu G et al. Opposite roles of CCR2 and CX3CR1 macrophages in alkali-induced corneal neovascularization. Cornea 2009-06-01 [PMID: 19421039] (IF/IHC)
• Lee EY, Chung CH, Khoury CC et al. The monocyte chemoattractant protein-1/CCR2 loop, inducible by TGF-beta, increases podocyte motility and albumin permeability. Am J Physiol Renal Physiol 2009-07-01 [PMID: 19420107] (ICC/IF)
• Chun KR, Bae EM, Kim JK et al. Suppression of the lipopolysaccharide-induced expression of MARCKS-related protein (MRP) affects transmigration in activated RAW264.7 cells. Cell Immunol 2009-01-01 [PMID: 19246034] (FLOW)
• Brambilla R, Persaud T, Hu XC et al. Transgenic Inhibition of Astroglial NF-?B Improves Functional Outcome in Experimental Autoimmune Encephalomyelitis by Suppressing Chronic Central Nervous System Inflammation . J Immunol 182 2628-2640. 2009-01-01 [PMID: 19234157] (IF/IHC)
• D’Mello C, Le T, Swain MG. Cerebral Microglia Recruit Monocytes into the Brain in Response to Tumor Necrosis Factor Signaling during Peripheral Organ Inflammation ,J. Neurosci 29 2089 - 2102. 2009-01-01 [PMID: 19228962] (ICC/IF)
• Knerlich-Lukoschus F, Juraschek M, Blmer U et al. Force-dependent development of neuropathic central pain and time-related CCL2/CCR2 expression after graded spinal cord contusion injuries of the rat. J Neurotrauma 2008-05-01 [PMID: 18338959] (ICC/IF)
• Kanamaru Y, Pfirsch S, Aloulou M et al. Inhibitory ITAM Signaling by Fc RI-FcR Chain Controls Multiple Activating Responses and Prevents Renal Inflammation . Journal of Immunology 180 2669-2678. 2008-01-01 [PMID: 18250479] (WB)
• Burt D, Salvidio G, Tarabra E et al. The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system affects cell motility in cultured human podocytes. Am J Pathol 2007-12-01 [PMID: 18055544] (ICC/IF)
• Tugizov S, Herrera R, Veluppillai P et al. Epstein-Barr virus (EBV)-infected monocytes facilitate dissemination of EBV within the oral mucosal epithelium. J Virol 2007-06-01 [PMID: 17376918] (WB)

Reviews for CCR2 Antibody (NB110-55674) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer WB Human 06/16/2017

Summary

• Application: Western Blot
• Sample Tested: HASMC human aortic smooth muscle cells
• Species: Human

reviewed by: Donatella Valdembri WB Mouse 09/19/2013

Summary

• Application: Western Blot
• Sample Tested: Mouse spleen, mouse liver, mouse bone marrow
• Species: Mouse
• Lot: YJ092910CS

FAQs for CCR2 Antibody (NB110-55674). (Showing 1 - 2 of 2 FAQs).

1. Could you please tell me the concentration of your CCR2 unconjugated E68 antibody?
   • Our lab has just confirmed for me that we currently have two vials of lot GR179302-2 in stock, which is at a concentration of 0.807mg/ml.
2. I am looking for the antibody concentration of the ccr2 antibody (NB110-55674). Where can I find that information?
   • The current production of our CCR2 antibody with catalogue number NB110-55674 has lot number GR179302-6, and is supplied at a concentration of 0.807mg/ml.

Bioinformatics

• Gene Symbol: CCR2
• Entrez:
  • 729230(Human)
  • 12772(Mouse)
  • 60463(Rat)
• Uniprot:
  • P41597(Human)