CCL7/MCP-3/MARC Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL7/MCP-3/MARC Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse CCL7/MCP-3/MARC (R&D Systems, Catalog # 456-MC)
  Gln24-Pro97
  Accession # Q03366
• Specificity: Detects mouse CCL7/MCP-3/MARC in Western blots. In this format, approximately 25% cross-reactivity with recombinant human (rh) Eotaxin is observed and less than 5% cross-reactivity with recombinant mouse (rm) Eotaxin, rmJE, rhMCP-1, rhMCP-2, and rhMCP-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL7/MCP-3/MARC Antibody [Biotin]

• C-C motif chemokine 7
• CCL7
• chemokine (C-C motif) ligand 7
• FIC
• MARC
• MCP-3
• MCP3Monocyte chemotactic protein 3
• MCP-3Small-inducible cytokine A7
• MGC138465
• Monocyte chemoattractant protein 3
• NC28SCYA6
• SCYA7MGC138463
• small inducible cytokine A7 (monocyte chemotactic protein 3)

Background

Mouse MARC, a member of the beta subfamily of chemokines, was initially identified as a transcript that is induced in a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Mouse MARC/MCP-3 expression has also been detected during murine experimental allergic encephalomyelitis in the spinal cord, and in LPS-stimulated murine WEHI -3 cells and Swiss 3T3 cells where MARC expression is glucocorticoid-attenuated. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor-activated gene. The mouse MARC cDNA encodes a 97 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 74 amino acid residue mature protein. Mouse CCR2, a mouse chemokine receptor, has been shown to bind JE/MCP-1 with high affinity and MARC/MCP-3 with lower affinity.

1. Kulmburg, P.A. et al. (1992) J. Exp. Med. 176:1773.
2. Thirion, S. et al. (1994) Biochem. Biophys. Res. Commun. 201:493.
3. Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756.
4. Kurihara, T. and R. Bravo (1996) J. Biol. Chem. 271:11603.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL7/MCP-3/MARC Antibody (BAF456)(1)

Publication using BAF456

• Carrillo A, Monreal Y, Ramirez P, Marin L, Parrilla P, Oliver FJ, Yelamos J Transcription regulation of TNF-alpha-early response genes by poly(ADP-ribose) polymerase-1 in murine heart endothelial cells. Nucleic Acids Res., 2004-02-03;32(2):757-66. 2004-02-03 [PMID: 14762203] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: CCL7
• Uniprot:
  • Q03366()