Recombinant Rat CCL4/MIP-1 beta Protein Summary
A biologically active protein to CCL4.
Source: E. coli.Amino Acid Sequence: APIGSDPPTS CCFSYTSRKI HRNFVMDYYE TSSLCSQPAV VFLTKKGRQI CADPSEPWVN EYVNDLELN
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
|Details of Functionality
CCL4 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-1000 ng/ml.
| Protein/Peptide Type
Biologically Active Protein
>95% pure by SDS-PAGE
Less than 1EU/ug of endotoxin as determined by LAL method
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4, 3 % trehalose.
>95% pure by SDS-PAGE
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Recombinant Rat CCL4/MIP-1 beta Protein
- C-C motif chemokine 4
- chemokine (C-C motif) ligand 4
- G-26 T-lymphocyte-secreted protein
- Lymphocyte activation gene 1 protein
- Macrophage inflammatory protein 1-beta
- MIP1 beta
- MIP-1 beta
- MIP-1 beta/CCL4
- PAT 744
- Protein H400
- secreted protein G-26
- small inducible cytokine A4 (homologous to mouse Mip-1b)
- Small-inducible cytokine A4
- T-cell activation protein 2
Chemokine (C-C motif) ligand 4 encoded by the CCL4 gene, also known as macrophage inflammatory protein-1beta (MIP-1beta) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant rat CCL4 contains 69 amino acids and it shares 80 % and 86 % a.a. sequence identity with human and murine CCL4. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 2 years from date of receipt.
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