CCL3L1/LD78 beta Antibody - BSA Free

Western Blot: LD78 beta Antibody [NBP1-42775] - Analysis of recombinant swine CCL3L1 at 7.8kDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with , Inc. Anti-swine CCL3L1. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.

Western blot using protein-A purified anti-swine CCL3L1 antibody shows detection of recombinant swine CCL3L1 at 7.8kDa raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with , Inc. Anti-swine CCL3L1. After washing, membrane was probed with Dylight (TM) 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody ().

• Reactivity: Po, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: LYOPH

CCL3L1/LD78 beta Antibody - BSA Free Summary

• Description: This product was Protein-A purified from monospecific antiserum by chromatography
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This protein-A purified CCL3L1/LD78 beta Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 70 amino acids of the mature swine CCL3L1/LD78 beta/LD78 beta protein. (Uniprot: A1YM34)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCL3L1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1 ug/ml
• Application Notes: This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kDa in size corresponding to swine CCL3L1 protein by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with CCL3L1/LD78 beta from bovine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against CCL3L1/LD78 beta from cat, dog, human, chimpanzee, baboon, bovine, and rat based on greater than 90% sequence homology. Cross-reactivity with CCL3L1/LD78 beta from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for CCL3L1/LD78 beta Antibody - BSA Free

• C-C motif chemokine 3-like 1
• CCL3L3
• chemokine (C-C motif) ligand 3-like 1
• D17S1718
• G0/G1 switch regulatory protein 19-2
• G0S19-2LD78
• LD78BETA
• LD78-beta(1-70)
• MGC104178
• MGC12815
• MGC182017
• MIP1AP
• PAT 464.2
• SCYA3L
• SCYA3L1464.2
• small inducible cytokine A3-like 1
• Small-inducible cytokine A3-like 1
• Tonsillar lymphocyte LD78 beta protein

Background

Chemokine (C-C motif) ligand 3-like 1 (CCL3L1), also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha; other synonyms: ld78beta, scya3l1, small inducible cytokine a3-like 1), is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. In humans, CCL3L1 is encoded by a variable copy-number gene. The CC family induces MCP1 and RANTES, and exhibits a variety of proinflammatory activities including chemotaxis, and functional and proliferative activation of leukocytes, lymphocytes, and macrophages. Its signal is transmitted through transmembrane receptors, CC chemokine receptors, CCR1, CCR3 and CCR5.
CCL3L1/MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils. CCL3L1 binds to several chemokine receptors. In humans these receptors include chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. In swine, CCL3L1 protein is 86% similar to CCL4 protein, and 73% similar to CCL5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL3L1
• Uniprot:
  • A1YM34(Porcine)