CCL3/MIP-1 alpha Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL3/MIP-1 alpha Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse CCL3/MIP-1 alpha (R&D Systems, Catalog # 450-MA)
  Ala24-Ala92
  Accession # Q5QNW0
• Specificity: Detects mouse MIP-1 alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with rhCCL3, rmCCL9/10 and rmCCL4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse CCL3/MIP-1 alpha Antibody (Catalog # AF-450-NA)
  ELISA Detection: Mouse CCL3/MIP-1 alpha Biotinylated Antibody (Catalog # BAF450)
  Standard: Recombinant Mouse CCL3/MIP-1 alpha (Catalog # 450-MA)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL3/MIP-1 alpha Antibody [Biotin]

• C-C motif chemokine 3
• MIP1-(a)
• AI323804
• CCL3
• chemokine (C-C motif) ligand 3
• G0S19-1
• LD78a
• LD78alpha
• MIP1 alpha
• MIP-1 alpha
• MIP1A
• MIP-1alpha
• MIP1-alpha
• PAT 464.1
• SCYA3
• SIS-beta

Background

The macrophage inflammatory proteins 1 alpha and 1 beta , two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP-1 alpha shares approximately 77% and 70% amino acid identity with human MIP-1 alpha and mouse MIP-1 beta , respectively. MIP-1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP-1 proteins are members of the beta (C-C) subfamily of chemokines.

Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8⁺ and CD4⁺ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha  has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, MIP-1 beta was reported to be much less active. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.

1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL3/MIP-1 alpha Antibody (BAF450)(12)

Publications using BAF450

• Katarzyna Kuczkowska, Geir Mathiesen, Vincent G. H. Eijsink, Inger Øynebråten Lactobacillus plantarum displaying CCL3 chemokine in fusion with HIV-1 Gag derived antigen causes increased recruitment of T cells Microbial Cell Factories 2015-12-01 [PMID: 26494531]
• Oynebraten I, Hinkula J, Fredriksen A, Bogen B Increased generation of HIV-1 gp120-reactive CD8+ T cells by a DNA vaccine construct encoding the chemokine CCL3. PLoS ONE, 2014-08-14;9(8):e104814. 8/14/2014 [PMID: 25122197]
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines. Am. J. Pathol., 2011-10-01;179(6):2730-9. 2011-10-01 [PMID: 21967814] (ELISA Development, Mouse)
• Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J. Immunol., 2009-10-07;183(9):5799-806. 2009-10-07 [PMID: 19812208] (ELISA Development, Mouse)
• Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). J. Biol. Chem., 2009-07-10;0(0):. 2009-07-10 [PMID: 19592492] (ELISA Development, Mouse)
• Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4). Eur. J. Immunol., 2006-08-01;36(8):2025-34. 2006-08-01 [PMID: 16856209] (ELISA Development, Mouse)
• Ellaban E, Bolgos G, Remick D Selective macrophage suppression during sepsis. Cell. Immunol., 2005-02-26;231(1):103-11. 2005-02-26 [PMID: 15919375] (ELISA Development, Mouse)
• Mosmann T Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions. Eur. J. Immunol., 2004-06-01;34(6):1617-26. 2004-06-01 [PMID: 15162431] (ELISA Development, ELISpot Development, Mouse)
• Reading PC, Symons JA, Smith GL A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection. J. Immunol., 2003-02-01;170(3):1435-42. 2003-02-01 [PMID: 12538705] (ELISA Development, Human)
• Horner AA, Datta SK, Takabayashi K, Belyakov IM, Cinman N, Nguyen MD, Van Uden JH, Berzofsky JA Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites. J. Immunol., 2001-08-01;167(3):1584-91. 2001-08-01 [PMID: 11466380] (ELISpot Development, Mouse)
• Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA Regulatory effects of eotaxin on acute lung inflammatory injury. J. Immunol., 2001-04-15;166(8):5208-18. 2001-04-15 [PMID: 11290805] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: CCL3
• Uniprot:
  • Q5QNW0()