CCL3/MIP-1 alpha Antibody (39624) [Unconjugated]

J774A.1 cells treated with 1µg/mL LPS and monensin for 5 hrs (filled histogram) and resting J774A.1 cells (open histogram) were stained with Rat Anti-Mouse CCL3/MIP‑1 alpha Monoclonal Antibody (Catalog # MAB450) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Mu
• Applications: WB, Flow
• Clone: 39624
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCL3/MIP-1 alpha Antibody (39624) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CCL3/MIP-1 alpha
  Ala24-Ala92
  Accession # P10855
• Specificity: Detects mouse CCL3/MIP‑1 alpha in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CCL4/
  MIP‑1 beta is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 6, 7, 9/10/MIP-1 gamma , 11, 12, 19, 21, 22, 25, 28, recombinant rat CCL2 or CCL20 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCL3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL3/MIP-1 alpha Antibody (39624) [Unconjugated]

• C-C motif chemokine 3
• MIP1-(a)
• AI323804
• CCL3
• chemokine (C-C motif) ligand 3
• G0S19-1
• LD78a
• LD78alpha
• MIP1 alpha
• MIP-1 alpha
• MIP1A
• MIP-1alpha
• MIP1-alpha
• PAT 464.1
• SCYA3
• SIS-beta

Background

The macrophage inflammatory proteins 1 alpha and 1 beta , two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse CCL3/MIP-1 alpha shares approximately 77% and 70% amino acid identity with human CCL3/MIP-1 alpha and mouse CCL4/MIP-1 beta , respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP‑1 proteins are members of the beta  (C‑C) subfamily of chemokines.

Both CCL3 and CCL4 are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with CCL3 and CCL4 preferentially attracting CD8⁺ and CD4⁺ T cells, respectively. CCL3 has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells, and CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, CCL4 was reported to be much less active. The functional receptor for CCL3 has been identified as CCR1 and CCR5.

1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL3/MIP-1 alpha Antibody (MAB450)(14)

Publications using MAB450

• MS Abdel-Hake, S Manne, JC Beltra, E Stelekati, Z Chen, K Nzingha, MA Ali, JL Johnson, JR Giles, D Mathew, AR Greenplate, G Vahedi, EJ Wherry Epigenetic scarring of exhausted T cells hinders memory differentiation upon eliminating chronic antigenic stimulation Nature Immunology, 2021-07-26;22(8):1008-1019. 2021-07-26 [PMID: 34312545] (Flow Cytometry, Mouse)
• R Braathen, HCL Spång, DM Hinke, J Blazevski, S Bobic, E Fossum, B Bogen A DNA Vaccine That Encodes an Antigen-Presenting Cell-Specific Heterodimeric Protein Protects against Cancer and Influenza Mol Ther Methods Clin Dev, 2020-01-25;17(0):378-392. 2020-01-25 [PMID: 32128342] (ELISA Capture, Mouse)
• Pratik P. Vikhe, Tom Purnell, Steve D.M. Brown, Derek W. Hood Cellular content plays a crucial role in Non-typeable Haemophilus influenzae infection of preinflamed Junbo mouse middle ear Cellular Microbiology 2019-01-01 [PMID: 30265765]
• Lauren A Jordan, Malin C Erlandsson, Benjamin F Fenner, Ruth Davies, Ann K Harvey, Ernest H Choy et al. Inhibition of CCL3 abrogated precursor cell fusion and bone erosions in human osteoclast cultures and murine collagen-induced arthritis Rheumatology (Oxford) 2018-11-01 [PMID: 30053130]
• Elena Farmaki Induction of the MCP chemokine cluster cascade in the periphery by cancer cell-derived CCL3 Cancer Lett, 2016-12-29;0(0):. 2016-12-29 [PMID: 28041977] (Neutralization, Mouse)
• Immune signatures of protective spleen memory CD8 T cells Sci Rep, 2016-11-24;6(0):37651. 2016-11-24 [PMID: 27883012] (Flow Cytometry, Mouse)
• Mechanisms of Invariant NKT Cell Activity in Restraining Bacillus anthracis Systemic Dissemination J Immunol, 2016-09-07;0(0):. 2016-09-07 [PMID: 27605012] (Neutralization, Mouse)
• Oynebraten I, Hinkula J, Fredriksen A, Bogen B Increased generation of HIV-1 gp120-reactive CD8+ T cells by a DNA vaccine construct encoding the chemokine CCL3. PLoS ONE, 2014-08-14;9(8):e104814. 8/14/2014 [PMID: 25122197]
• Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse)
• Deepak P, Kumar S, Kishore D, Acharya A IL-13 from Th2-type cells suppresses induction of antigen-specific Th1 immunity in a T-cell lymphoma. Int. Immunol., 2009-11-30;22(1):53-63. 2009-11-30 [PMID: 19951958] (ELISA Development, Mouse)
• Fujita H, Asahina A, Komine M, Tamaki K The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. Cell. Immunol., 2007-05-15;245(2):70-9. 2007-05-15 [PMID: 17507001] (Neutralization, Mouse)
• Mosmann T Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions. Eur. J. Immunol., 2004-06-01;34(6):1617-26. 2004-06-01 [PMID: 15162431] (ELISA Development, Mouse)
• Reading PC, Symons JA, Smith GL A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection. J. Immunol., 2003-02-01;170(3):1435-42. 2003-02-01 [PMID: 12538705] (ELISA Development, Mouse)
• Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA Regulatory effects of eotaxin on acute lung inflammatory injury. J. Immunol., 2001-04-15;166(8):5208-18. 2001-04-15 [PMID: 11290805] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: CCL3
• Uniprot:
  • P10855()