Mouse CCL24/Eotaxin-2/MPIF-2 ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard Curve. Ccl24 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Ccl24. |
| Sensitivity |
4 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
CCL24 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
| Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Kit Components
|
Components
|
- Assay Diluent A (Item D): Diluents buffer, 0.09% sodium azide as preservative.
- Assay Diluent B (Item E): 5x concentrated buffer.
- Detection Antibody Eotaxin-2 (Item F): Biotinylated anti-mouse Eotaxin-2. Each vial isenough to assay half the microplate.
- Eotaxin-2 Microplate (Item A): 96 wells coated with anti-mouse Eotaxin-2.
- HRP-Streptavidin Concentrate (Item G): 200x concentrated HRP-conjugatedstreptavidin.
- Note: Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
- Standard protein (Item C): Mouse Eotaxin-2. 1 vial is enough to run each standard induplicate.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3,3’,5,5’-tetramethylbenzidine (TMB) inbuffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mouse CCL24/Eotaxin-2/MPIF-2 ELISA Kit (Colorimetric)
Background
Eotaxin 2, also known as CCL24, is a part of a subfamily of the cytokine CC genes which are involved in immunoregulatory and inflammatory processes. Eotaxin 2 plays a role inducing chemotaxis in eosinophils when interacting with CCR3. This protein is known to have interactions with CCR3, CCR1, CCR10, CCR2 and CCR4.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for CCL24/Eotaxin-2/MPIF-2 ELISA Kit (KA1782). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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