CCL24/Eotaxin-2/MPIF-2 Antibody (106521) [Unconjugated]

Recombinant Mouse CCL24/Eotaxin‑2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin‑2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL24/Eotaxin‑2 Monoclonal Antibody (Catalog # MAB528). The ND50 is typically 0.06‑0.3 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 106521
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCL24/Eotaxin-2/MPIF-2 Antibody (106521) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2
  Ile24-Val119
  Accession # Q9JKC0
• Specificity: Detects mouse CCL24/Eotaxin‑2/MPIF‑2 in Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10/MIP-1 gamma , 11, 12, 19, 20, 21, 22, 25, 28, or recombinant rat CCL20 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCL24
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.06-0.3 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse CCL24/Eotaxin-2/MPIF-2 Biotinylated Antibody (Catalog number BAF528)
  Standard: Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 aa 24-119 (Catalog number 528-MP)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL24/Eotaxin-2/MPIF-2 Antibody (106521) [Unconjugated]

• CCL24
• chemokine (C-C motif) ligand 24
• Ckb-6
• Eosinophil chemotactic protein 2
• Eotaxin-2
• member 24
• MPIF2
• MPIF-2
• MPIF2Myeloid progenitor inhibitory factor 2
• MPIF-2Small-inducible cytokine A24
• SCYA24CK-beta-6

Background

Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to CCL11/Eotaxin and CCL26/Eotaxin-3. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by Eotaxin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL24/Eotaxin-2/MPIF-2 Antibody (MAB528)(8)

Publications using MAB528

• Kristen L. Mittelsteadt, Erika T. Hayes, Daniel J. Campbell ICOS signaling limits regulatory T cell accumulation and function in visceral adipose tissue Journal of Experimental Medicine 6/7/2021 [PMID: 33881452]
• Sonia Ventura, Florencia Cano, Yashaswini Kannan, Felix Breyer, Michael J. Pattison, Mark S. Wilson et al. A20-binding inhibitor of NF-kappa B (ABIN) 2 negatively regulates allergic airway inflammation Journal of Experimental Medicine 11/5/2018 [PMID: 30337469]
• Y Kannan, LJ Entwistle, VS Pelly, J Perez-Llor, AW Walker, SC Ley, MS Wilson TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus PLoS Pathog., 2017-07-31;13(7):e1006536. 2017-07-31 [PMID: 28759611] (Neutralization, Mouse)
• AJ Byrne, M Weiss, SA Mathie, SA Walker, HL Eames, D Saliba, CM Lloyd, IA Udalova A critical role for IRF5 in regulating allergic airway inflammation Mucosal Immunol, 2016-10-19;10(3):716-726. 2016-10-19 [PMID: 27759022] (ELISA Development (Capture), Mouse)
• Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function. Immunity, 2015-11-17;43(5):945-58. 2015-11-17 [PMID: 26588780] (ELISA Development (Capture), Mouse)
• Cuilian Dai, Xianglan Yao, Elizabeth M. Gordon, Amisha Barochia, Rosemarie A. Cuento, Maryann Kaler et al. A CCL24-dependent pathway augments eosinophilic airway inflammation in house dust mite-challenged Cd163(-/-) mice Mucosal Immunology 5/1/2016 [PMID: 26376364]
• Wakashin H, Hirose K, Maezawa Y, Kagami S, Suto A, Watanabe N, Saito Y, Hatano M, Tokuhisa T, Iwakura Y, Puccetti P, Iwamoto I, Nakajima H IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice. Am. J. Respir. Crit. Care Med., 2008-09-11;178(10):1023-32. 2008-09-11 [PMID: 18787221] (Neutralization, Mouse)
• Shibamori M, Ogino K, Kambayashi Y, Ishiyama H Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice. Life Sci., 2005-10-17;78(9):987-94. 2005-10-17 [PMID: 16229861] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: CCL24
• Uniprot:
  • Q9JKC0()