CCL23/Ck beta 8-1/MIP3 Antibody (103952) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB, ELISA(Cap), ELISA(Det)
• Clone: 103952
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

CCL23/Ck beta 8-1/MIP3 Antibody (103952) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human CCL23/CKβ8-1
  Met46-Asn137
  Accession # AAD10846
• Specificity: Detects human CCL23/Ck beta 8‑1 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity with human CCL23/CK beta 8 isoform is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL23/Ck beta 8-1/MIP3 Antibody (103952) [Alexa Fluor® 750]

• C6 beta-chemokine
• CCL23/Ck beta 8-1
• chemokine (C-C motif) ligand 23
• Ck beta 8-1
• CKb8
• Ckb-8
• Ckb81
• CK-beta-8
• hmrp-2a
• Macrophage inflammatory protein 3
• MIP3
• MIP-3CK-BETA-8
• MPIF1
• MPIF-1Ckb-8-1
• Myeloid progenitor inhibitory factor 1
• SCYA23C-C motif chemokine 23
• small inducible cytokine subfamily A (Cys-Cys), member 23
• Small-inducible cytokine A23

Background

CCL23 (SCY A23), a splice variant of CK beta 8 (also known as MPIF-1), is a beta chemokine isolated from the THP-1 cDNA library. CCL23 cDNA encodes a 137 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide. Compared to CK beta 8-1, CK beta 8/MPIF-1 lacks a 17 aa residue stretch (Leu47‑Gly63) present in CCL23 and has a unique arginine at residue 46. CK beta 8-1/CK beta 8 (MPIF-1) and leukotactin/MIP-1δ form a subgroup of beta chemokines that have 6 conserved cysteine residues and an extended amino-terminus preceding the conserved cysteine pair. CCL23 shares approximately 73% nucleotide sequence identity with leukotactin (MIP-1δ), and the two genes have been localized to human chromosome 17 within 200 kb of each other. CCL23 mRNA expression has been detected in the pancreas, heart and skeletal muscle. Both CK beta 8-1 and CK beta 8 (MPIF-1) are potent agonists of CCR1 and have been shown to chemoattract peripheral blood lymphocytes and monocytes. The two chemokines have also been reported to chemoattract neutrophils and to inhibit colony formation by human hematopoietic progenitor cells. Similar to CK beta 8 (MPIF-1), the truncated form of CK beta 8-1 (aa 46‑132) produced at R&D Systems has been shown to have greatly enhanced biological activity compared to the full length mature CK beta 8-1 (aa 22‑132).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.