CCL22/MDC Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL22/MDC Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse CCL22/MDC (R&D Systems, Catalog # 439-MD)
  Gly25-Ser92
  Accession # Q9QZU2
• Specificity: Detects mouse MDC in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity recombinant human (rh) MDC, recombinant mouse (rm) 6Ckine, rmFractalkine, and rmTARC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL22
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Mouse CCL22/MDC Antibody (Catalog # MAB4391)
  ELISA Detection: Mouse CCL22/MDC Biotinylated Antibody (Catalog # BAF439)
  Standard: Recombinant Mouse CCL22/MDC (Catalog # 439-MD)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca²⁺ mobilization in activated mouse and human T cells.

1. Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
2. Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
3. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
4. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL22