CCL22/MDC Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Biotin
• Concentration: LYOPH

CCL22/MDC Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CCL22/MDC (R&D Systems, Catalog # 336-MD)
  Gly25-Gln93
  Accession # O00626
• Specificity: Detects human CCL22/MDC in ELISAs and Western blots. In sandwich immunoassays, less than 0.03% cross-reactivity with recombinant mouse TARC, recombinant human (rh) TARC, and rh6Ckine is observed.
• Source: N/A
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: CCL22
• Purity Statement: Antigen Affinity-purified from egg yolks

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human CCL22/MDC Antibody (Catalog # MAB336)
  ELISA Detection: Human CCL22/MDC Biotinylated Antibody (Catalog # BAF336)
  Standard: Recombinant Human CCL22/MDC (Catalog # 336-MD)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL22/MDC Antibody [Biotin]

• A-152E5.1
• ABCD-1
• CC chemokine STCP-1
• C-C motif chemokine 22
• CCL22
• chemokine (C-C motif) ligand 22
• DC/B-CK
• Macrophage-derived chemokine
• MDC
• MDCStimulated T-cell chemotactic protein 1
• MGC34554
• SCYA22MDC(1-69)
• small inducible cytokine A22
• small inducible cytokine subfamily A (Cys-Cys), member 22
• Small-inducible cytokine A22
• STCP-1
• stimulated T cell chemotactic protein 1

Background

CCL22, also named stimulated T cell chemotactic protein (STCP-1), is a CC chemokine initially isolated from clones of monocyte-derived macrophages. Human CCL22 cDNA encodes a precursor protein of 93 amino acid residues with a 24 amino acid residue predicted signal peptide that is cleaved to yield a 69 amino acid residue mature 8 kDa protein. At the amino acid sequence level, CCL22 shows less than 35% identity to other CC chemokine family members. Human CCL22 is expressed in dendritic cells, macrophages and activated monocytes. In addition, CCL22 expression is also detected in the tissues of thymus, lymph node and appendix. The gene for human CCL22 has been mapped to chromosome 16 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Recombinant or chemically synthesized mature CCL22 has been shown to induce chemotaxis or Ca²⁺ mobilization in dendritic cells, IL-2 activated NK cells, and activated T lymphocytes. A CD8⁺ T lymphocyte-derived secreted soluble activity that suppresses infection by primary non-syncytium-inducing and syncytium-inducing HIV-1 isolates and the T-cell line-adapted isolate HIV-1_IIIB, has been identified as CCL22. Based on amino-terminal sequence analysis, the major CD8⁺ T lymphocyte-derived CCL22 protein yielded an amino-terminal sequence of YGANM, which is two amino acid residues shorter than the predicted mature CCL22. The difference in potency between the two mature CCL22 isoforms has not been determined.

1. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
2. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.
3. Pal, R. et al. (1997) Science 278:5338.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL22/MDC Antibody (BAF336)(7)

Publications using BAF336

• C Kötting, L Hofmann, R Lotfi, D Engelhardt, S Laban, PJ Schuler, TK Hoffmann, C Brunner, MN Theodoraki Immune-Stimulatory Effects of Curcumin on the Tumor Microenvironment in Head and Neck Squamous Cell Carcinoma Cancers, 2021-03-16;13(6):. 2021-03-16 [PMID: 33809574] (ELISA Detection, Human)
• Martina Sandberg Abelius, Uta Enke, Frauke Varosi, Heike Hoyer, Ekkehard Schleussner, Maria C. Jenmalm et al. Placental immune response to apple allergen in allergic mothers Journal of Reproductive Immunology 12/1/2014 [PMID: 25048667]
• T. R. Abrahamsson, M. Sandberg Abelius, A. Forsberg, B. Björkstén, M. C. Jenmalm A Th1/Th2-associated chemokine imbalance during infancy in children developing eczema, wheeze and sensitization Clinical & Experimental Allergy 12/1/2011 [PMID: 21801246]
• Catrin Furuhjelm, Maria C Jenmalm, Karin Fälth-Magnusson, Karel Duchén Th1 and Th2 Chemokines, Vaccine-Induced Immunity, and Allergic Disease in Infants After Maternal omega -3 Fatty Acid Supplementation During Pregnancy and Lactation Pediatric Research 3/1/2011 [PMID: 21099447]
• Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha. J. Immunol., 2007-10-01;179(7):4840-8. 2007-10-01 [PMID: 17878383] (ELISA Development, Human)
• Liu L, Jarjour NN, Busse WW, Kelly EA Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma. Am. J. Respir. Crit. Care Med., 2004-03-04;169(10):1118-24. 2004-03-04 [PMID: 15001464] (ELISA Development, Human)
• Conroy DM, Jopling LA, Lloyd CM, Hodge MR, Andrew DP, Williams TJ, Pease JE, Sabroe I CCR4 blockade does not inhibit allergic airways inflammation. J. Leukoc. Biol., 2003-07-15;74(4):558-63. 2003-07-15 [PMID: 12960268] (ELISA Development, Guinea Pig)

Reviews for CCL22/MDC Antibody (BAF336) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer ELISA Human 01/13/2015

Summary

• Application: ELISA
• Sample Tested: See PMID 21801246
• Species: Human

reviewed by: Verified Customer ELISA Human 01/13/2015

Summary

• Application: ELISA
• Sample Tested: See PMID 23517039
• Species: Human

Bioinformatics

• Gene Symbol: CCL22
• Uniprot:
  • O00626()