CCL21/6Ckine Antibody (709408R) [Unconjugated]

Recombinant Mouse CCL21/6Ckine (Catalog # 457-6C) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL21/6Ckine (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB4572). The ND50 is typically 0.5-3 µg/mL.

• Reactivity: Mu
• Applications: B/N
• Clone: 709408R
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCL21/6Ckine Antibody (709408R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant mouse CCL21/6Ckine
  Accession # P84444
• Specificity: Detects mouse CCL21/6Ckine in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.5-3 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL21/6Ckine Antibody (709408R) [Unconjugated]

• 6Ckine
• 6CkineSmall-inducible cytokine A21
• Beta-chemokine exodus-2
• CCL21
• chemokine (C-C motif) ligand 21
• CKb9
• exodus-2
• member 21
• SCYA21
• SCYA21MGC34555
• secondary lymphoid tissue chemokine
• SLC
• SLCSecondary lymphoid-tissue chemokine
• TCA-4

Background

6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoid-tissue chemokine), CCL21 and Exodus-2, shows 21‑33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid highly basic precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine share 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells. Recombinant human 6Ckine has been shown to be chemotactic for some human T cell lines, resting PBL, and cultured T cells expanded with PHA and IL-2. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via the CC receptor CCR7 on T cells and B cells.

1. Hedrick, J.A. and A. Zlotnik (1997) J. Immunol. 159:1589.
2. Hromas, R. et al. (1997) J. Immunol. 159:2554.
3. Nagira, M. et al. (1997) J. Biol. Chem. 272:19518.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL21/6Ckine Antibody (MAB4572)(1)

Publication using MAB4572

• A Jarade, Z Garcia, S Marie, A Demera, I Prinz, P Bousso, JP Di Santo, N Serafini Inflammation triggers ILC3 patrolling of the intestinal barrier Nature Immunology, 2022-08-23;0(0):. 2022-08-23 [PMID: 35999393] (In Vivo, Mouse)

Bioinformatics

• Uniprot:
  • P84444()