Recombinant Porcine CCL2/MCP1 Protein

ELISA: Recombinant Porcine CCL2/MCP1 Protein [NBP2-62499] - Pig MCP-1 ELISA. Recombinant Pig MCP-1 detected using Rabbit anti Pig MCP-1 as the capture reagent and biotinylated Rabbit anti Pig MCP-1 antibody as the detection reagent followed by Streptavidin:HRP

• Reactivity: Po
• Applications: WB, ELISA
• Concentration: LYOPH

Recombinant Porcine CCL2/MCP1 Protein Summary

• Description: Recombinant pig MCP-1.
  
  

  Source:Pichia pastoris

  Amino Acid Sequence:aa 24 - 99 of porcine monocyte chemotactic protein 1 (MCP-1), UniProt P42831

• Preparation Method: Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography.
• Source: Pichia pastoris
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL2
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Theoretical MW: 8.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 10% Trehalose
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with 20 ul sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix vial after reconstitution.

Alternate Names for Recombinant Porcine CCL2/MCP1 Protein

• C-C motif chemokine 2
• chemokine (C-C motif) ligand 2
• GDCF-2
• HC11Monocyte secretory protein JE
• MCAFMonocyte chemotactic and activating factor
• MCP1 Monocyte chemotactic protein 1
• MCP-1 Small-inducible cytokine A2
• MGC9434
• Monocyte chemoattractant protein 1
• monocyte chemoattractant protein-1
• SCYA2HSMCR30
• small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
• small inducible cytokine subfamily A (Cys-Cys), member 2
• SMC-CF

Background

Monocyte Chemoattracctant Protein-1 (MCP1), also known as CCL2, is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli and is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. MCP1 augments monocyte anti-tumor activity and has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. In atherosclerosis MCP-1 has been implicated to be involved in the recruitment of monocytes into the arterial wall during the disease process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CCL2
• Uniprot:
  • P42831(Porcine)