Mouse CCL2/MCP1 ELISA Kit (Colorimetric)

Mouse CCL2/MCP1 ELISA Kit [NBP1-92659] - Data for Mouse MCP-1 Platinum ELISA

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum
• Standard Curve Range: 15.6 - 1,000 pg/mL
• Sensitivity: 2.2 pg/mL

Mouse CCL2/MCP1 ELISA Kit (Colorimetric) Summary

• Description: The mouse MCP-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse MCP-1
• Specificity: The assay detects both natural and recombinant mouse MCP-1. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse MCP-1 positive serum. There was no crossreactivity detected, notably not with TCA3 and MIP-1 beta.
• Standard Curve Range: 15.6 - 1,000 pg/mL
• Sensitivity: 2.2 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.60%
• Intra-Assay: CV% < 6.20%
• Spike Recovery: The recovery ranged from 80% to 111% with an overall mean recovery of 98%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CCL2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse CCL2/MCP1 ELISA Kit (Colorimetric)

• C-C motif chemokine 2
• chemokine (C-C motif) ligand 2
• GDCF-2
• HC11Monocyte secretory protein JE
• MCAFMonocyte chemotactic and activating factor
• MCP1 Monocyte chemotactic protein 1
• MCP-1 Small-inducible cytokine A2
• MGC9434
• Monocyte chemoattractant protein 1
• monocyte chemoattractant protein-1
• SCYA2HSMCR30
• small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
• small inducible cytokine subfamily A (Cys-Cys), member 2
• SMC-CF

Background

MCP-1was characterized as a monocyte-specific chemoattractant that was later shown to attract also T lymphocytes and NK cells. MCP-1 is mainly expressed by macrophages in response to a wide range of cytokines such as IL-6, TNF-alpha and IL-1beta, but can, upon stimulation, also be produced by a variety of cells and tissues, such as fibroblasts, endothelial cells or certain tumor cells. Because of its target cell specificity, MCP-1 was postulated to play a pathogenic role in a variety of diseases characterized by mononuclear cell infiltration, including atherosclerosis, rheumatoid arthritis and allergic responses. In basophils, MCP-1 is highly effective as a stimulus of histamine release but has only weak chemotactic activity. Additionally it has been shown to chemoattract CD4+ and CD8+ T lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CCL2/MCP1 ELISA Kit (NBP1-92659). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CCL2