Human CCL2/MCP1 ELISA Kit (Colorimetric)

ELISA: CCL2/MCP1 ELISA Kit [NBP1-91276] - Standard curve

Product Discontinued

Human CCL2/MCP1 ELISA Kit (Colorimetric) Summary

• Description: This ELISA kit has been transitioned to a new version, available here: DCP00
• Specificity: The assay detects both natural and recombinant human MCP-1. The cross reactivity of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human MCP- 1 positive serum. There was no cross reactivity detected, namely not with MCP-3.
• Standard Curve Range: 15.6 - 1,000 pg/mL
• Sensitivity: 2.3 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 8.70%
• Intra-Assay: CV% < 4.70%
• Spike Recovery: The recovery ranged from 84% to 98% with an overall mean recovery of 92%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 20 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CCL2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CCL2/MCP1 ELISA Kit (Colorimetric)

• C-C motif chemokine 2
• chemokine (C-C motif) ligand 2
• GDCF-2
• HC11Monocyte secretory protein JE
• MCAFMonocyte chemotactic and activating factor
• MCP1 Monocyte chemotactic protein 1
• MCP-1 Small-inducible cytokine A2
• MGC9434
• Monocyte chemoattractant protein 1
• monocyte chemoattractant protein-1
• SCYA2HSMCR30
• small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
• small inducible cytokine subfamily A (Cys-Cys), member 2
• SMC-CF

Background

MCP-1was characterized as a monocyte-specific chemoattractant that was later shown to attract also T lymphocytes and NK cells. The human mature MCP-1 protein consists of 76 amino acids, derived by cleavage of a hydrophobic signal peptide from the 99 aa precursor protein. MCP-1 is mainly expressed by macrophages in response to a wide range of cytokines such as IL-6, TNF-alpha and IL-1beta, but can, upon stimulation, also be produced by a variety of cells and tissues, such as fibroblasts, endothelial cells or certain tumor cells. Because of its target cell specificity, MCP-1 was postulated to play a pathogenic role in a variety of diseases characterized by mononuclear cell infiltration, including atherosclerosis, rheumatoid arthritis and allergic responses. In basophils, MCP-1 is highly effective as a stimulus of histamine release but has only weak chemotactic activity. Additionally it has been shown to chemoattract CD4+ and CD8+ T lymphocytes, and expression of the MCP-1 chemokine may affect HIV infection via signaling through the CCR2 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CCL2/MCP1 ELISA Kit (NBP1-91276). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CCL2