CCL2/MCP1 Antibody

Images

 
Western Blot: CCL2/MCP1 Antibody [NBP1-07034] - Analysis of recombinant mouse MCP1. Image provided via product review by verified customer.
Western Blot: CCL2/MCP1 Antibody [NBP1-07034] - Analysis of MCP1 in Hela whole cell lysates.

Product Details

Summary
Reactivity Hu, Rt, MuSpecies Glossary
Applications WB, ICC/IF (-), IHC (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.42 mg/ml

CCL2/MCP1 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of rat MCP1 (within residues 30-80). [Swiss-Prot# P14844]
Localization
Secreted
Predicted Species
Mouse (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
Application Notes
In Western blot, a band is seen at ~16 kDa. This antibody did not work in IHC with mouse tissue or in ICC.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CCL2/MCP1 Overexpression Lysate
CCL2/MCP1 Knockout HeLa Cell Lysate
Control Peptide
CCL2/MCP1 Peptide (NBP1-07034PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-07034 in the following applications:

Publications
Read Publications using
NBP1-07034 in the following applications:

  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported by a customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.42 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CCL2/MCP1 Antibody

  • C-C motif chemokine 2
  • chemokine (C-C motif) ligand 2
  • GDCF-2
  • HC11Monocyte secretory protein JE
  • MCAFMonocyte chemotactic and activating factor
  • MCP1 Monocyte chemotactic protein 1
  • MCP-1 Small-inducible cytokine A2
  • MGC9434
  • Monocyte chemoattractant protein 1
  • monocyte chemoattractant protein-1
  • SCYA2HSMCR30
  • small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
  • small inducible cytokine subfamily A (Cys-Cys), member 2
  • SMC-CF

Background

MCP-1 (monocyte chemoattracctant protein-1, also called C-C motif chemokine 2 or CCL2) is a secreted protein belonging to intercrine beta (chemokine CC) family and it specifically act as a chemotactic factor to attracts monocytes, CD4+ T cells, NK cells as well as for CCR2 expressing cells, but not neutrophils. Both hematopoietic and non-hematopoietic cells that include monocytes/macrophages, dendritic cells, astrocytes, endothelial cells and fibroblasts express MCP-1, and monocytes/macrophages are the major source of MCP-1 in peripheral blood. Besides, expression at acute inflammation sites, MCP-1 is upregulated in chronic inflammation, including autoimmune disease, atherosclerosis, cancer, and chronic infection. MCP-1 has been implicated in both pro-inflammatory and anti-inflammatory responses, and is a potential intervention point for the treatment of various inflammatory/autoimmune diseases (multiple sclerosis, atherosclerosis, insulin-resistant diabetes, rheumatoid arthritis and lupus nephritis), cancers (breast and prostate cancer) and HIV-1/AIDS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB208
Species: Hu
Applications: WB, Simple Western, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
MAB150
Species: Hu
Applications: Flow, IHC, CyTOF-ready
NB100-56583
Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
MAB2005
Species: Hu
Applications: WB, Flow, CyTOF-ready
6507-IL/CF
Species: Hu
NBP1-90214
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for CCL2/MCP1 Antibody (NBP1-07034)(3)

Review for CCL2/MCP1 Antibody (NBP1-07034) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-07034:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot CCL2/MCP1 NBP1-07034
Enlarge
reviewed by:
David Jeng
WB Mouse 06/07/2013
View

Summary

ApplicationWestern Blot
Sample TestedRecombinant Mouse MPC1 protein
SpeciesMouse
CommentsThis antibody detected mouse MCP1 protein at the predicted size. According to R&D, mouse MCP1 should be approximately 8.5 kDa.

Blocking

Blocking Details5% non-fat dry milk in TBST for 1 hour

Primary Anitbody

Dilution Ratio1:1000 for 1 hour at room temperature

Secondary Antibody

Secondary Descriptionanti-rabbit HRP
Secondary Concentration1:10000

Details

Detection NotesDetected using ECL (Pierce west femto) and exposed to a CCD camera (Kodak)

Comments

CommentsThis antibody detected mouse MCP1 protein at the predicted size. According to R&D, mouse MCP1 should be approximately 8.5 kDa.

Product General Protocols

View specific protocols for CCL2/MCP1 Antibody (NBP1-07034): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CCL2/MCP1 Antibody (NBP1-07034). (Showing 1 - 2 of 2 FAQs).

  1. Our customer would like to perform IHC with samples from Rat. She has questions regarding antibodies of choice: 1. Is it possible to use NB400-142 for IHC? 2. I am having difficulty of choosing between NBP1-77679 and NBP1-77680. Would you please help suggest? 3. Although it is described as IHC (-) on the product page of NBP1-07034, This antibody did not work in IHC with mouse tissue is also described. How about Rat samples? Is it possible that the antibody can work in IHC with rat tissues?
    • To answer your questions about NB400-142, this antibody has not been validated for IHC and we cannot guarantee that it will work. Both NBP1-77679 and NBP1-77680 have been validated for use in rat and IHC. The difference between these 2 antibodies is the immunogen as they were raised against different regions of the protein. Please point out the immunogen to your customer and have them decide what would be best for their research. NBP1-07034 does not work in IHC, it is only guaranteed for Western blot in human, mouse and rat. When an application is followed by a (-) I would not recommend using this application in any species.
  2. Would you please let me know what buffer I should use for WB with antibody (MCP1 Antibody)?
    • In western blot, we recommend diluting this antibody in 1% non-fat dry milk in TBST (0.1% tween-20). This is the same diluent we use for our secondary antibody. For blocking, we use 5% non-fat dry milk in TBST for 1 hour at room temperature.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-07034AF350
Alexa Fluor 405 NBP1-07034AF405
Alexa Fluor 488 NBP1-07034AF488
Alexa Fluor 532 NBP1-07034AF532
Alexa Fluor 594 NBP1-07034AF594
Alexa Fluor 647 NBP1-07034AF647
Alexa Fluor 700 NBP1-07034AF700
Alexa Fluor 750 NBP1-07034AF750
Biotin NBP1-07034B
DyLight 350 NBP1-07034UV
DyLight 405 NBP1-07034V
DyLight 488 NBP1-07034G
DyLight 550 NBP1-07034R
DyLight 594 NBP1-07034DL594
DyLight 650 NBP1-07034C
DyLight 680 NBP1-07034FR
DyLight 755 NBP1-07034IR
FITC NBP1-07034F
HRP NBP1-07034H

Additional CCL2/MCP1 Products

Bioinformatics Tool for CCL2/MCP1 Antibody (NBP1-07034)

Discover related pathways, diseases and genes to CCL2/MCP1 Antibody (NBP1-07034). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CCL2/MCP1 Antibody (NBP1-07034)

Discover more about diseases related to CCL2/MCP1 Antibody (NBP1-07034).
 

Pathways for CCL2/MCP1 Antibody (NBP1-07034)

View related products by pathway.

PTMs for CCL2/MCP1 Antibody (NBP1-07034)

Learn more about PTMs related to CCL2/MCP1 Antibody (NBP1-07034).
 

Research Areas for CCL2/MCP1 Antibody (NBP1-07034)

Find related products by research area.

Blogs on CCL2/MCP1.

MCP-1 - chemoattractant protein involed in monocyte migration and infiltration
Monocyte chemotractant protein-1 (MCP-1), also known as CCL2, is a key chemokine involved in the migration of monocytes and macrophages to sites of active inflammation. It is a member of the C-C/beta family of cytokines, characterized by the Cys-C...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

David Jeng
06/07/2013
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CCL2