CCL2/JE/MCP-1 Antibody [Unconjugated]

479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (Catalog # 456-MC). PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." > 479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (Catalog # 456-MC). PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." title="Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (Catalog # 456-MC). PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." />

Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP-1 (Catalog # <a class=

479-JE) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA). The ND₅₀ is typically 10-30 µg/mL." > 479-JE) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA). The ND₅₀ is typically 10-30 µg/mL." title="Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA). The ND₅₀ is typically 10-30 µg/mL." />

Recombinant Mouse CCL2/JE (Catalog # <a class=

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL2/JE/MCP-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CCL2/JE/MCP-1
  Gln24-Arg96
  Accession # Q5SVU3
• Specificity: Detects mouse CCL2/JE/MCP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human MCP-1, recombinant mouse (rm MCP-5, and recombinant rat JE is observed. In Western blots, no cross-reactivity with rmEotaxin, rmMCP-5, or rmCCL7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ccl2
• Purity Statement: Protein A or G purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 10-30 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL2/JE/MCP-1 Antibody [Unconjugated]

• C-C motif chemokine ligand 2
• CCL2
• GDCF-2
• HC11
• HSMCR30
• MCAF
• Mcp1
• MCP-1
• SCYA2
• SMC-CF

Background

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

1. Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
2. Gu, L. et al. (1999) Chem. Immunol. 72:7.
3. Luini, W. et al. (1994) Cytokine 6:28.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL2/JE/MCP-1 Antibody (AB-479-NA)(13)

Publications using AB-479-NA

• Gregory P. Takacs, Christian J. Kreiger, Defang Luo, Guimei Tian, Julia S. Garcia, Loic P. Deleyrolle et al. Glioma-derived CCL2 and CCL7 mediate migration of immune suppressive CCR2+/CX3CR1+ M-MDSCs into the tumor microenvironment in a redundant manner Frontiers in Immunology 2023-01-04 [PMID: 36685592]
• Hyun-Jung Kim, Woo-Jin Kim, Hye-Rim Shin, Hee-In Yoon, Jae-I Moon, Eunji Lee et al. ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NF kappa B activation Cellular and Molecular Life Sciences 2022-03-01 [PMID: 35218410]
• Taito Miyamoto, Ryusuke Murakami, Junzo Hamanishi, Kenji Tanigaki, Yuko Hosoe, Nathan Mise et al. B7-H3 Suppresses Antitumor Immunity via the CCL2–CCR2–M2 Macrophage Axis and Contributes to Ovarian Cancer Progression Cancer Immunology Research 2022-01-01 [PMID: 34799346] (Flow Cytometry, Mouse)
• Ivan Flores, Steven S Welc, Michelle Wehling-Henricks, James G Tidball Myeloid cell-mediated targeting of LIF to dystrophic muscle causes transient increases in muscle fiber lesions by disrupting the recruitment and dispersion of macrophages in muscle Human Molecular Genetics 2021-12-27 [PMID: 34392367]
• X Liu, A Guo, Y Tu, W Li, L Li, W Liu, Y Ju, Y Zhou, A Sang, M Zhu Fruquintinib inhibits VEGF/VEGFR2 axis of choroidal endothelial cells and M1-type macrophages to protect against mouse laser-induced choroidal neovascularization Cell Death Dis, 2020-11-27;11(11):1016. 2020-11-27 [PMID: 33247124] (Cell Culture, Human)
• Itzel G. Ramírez-Moreno, Alfredo Ibarra-Sánchez, Jorge Ivan Castillo-Arellano, Ulrich Blank, Claudia González-Espinosa Mast Cells Localize in Hypoxic Zones of Tumors and Secrete CCL-2 under Hypoxia through Activation of L-Type Calcium Channels The Journal of Immunology 2020-02-15 [PMID: 31900336]
• SS Welc, I Flores, M Wehling-He, J Ramos, Y Wang, C Bertoni, JG Tidball Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy Nat Commun, 2019-06-26;10(1):2788. 2019-06-26 [PMID: 31243277] (IHC, Mouse, Transgenic Mouse)
• B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC Oncogene, 2019-02-14;0(0):. 2019-02-14 [PMID: 30765860] (Neutralization, Mouse)
• Yi Li, Yuhuan Zheng, Tianshu Li, Qiang Wang, Jianfei Qian, Yong Lu et al. Chemokines CCL2, 3, 14 stimulate macrophage bone marrow homing, proliferation, and polarization in multiple myeloma Oncotarget 2015-09-15 [PMID: 26155942]
• Julie Norseen, Tetsuya Hosooka, Ann Hammarstedt, Mark M. Yore, Shashi Kant, Pratik Aryal et al. Retinol-Binding Protein 4 Inhibits Insulin Signaling in Adipocytes by Inducing Proinflammatory Cytokines in Macrophages through a c-Jun N-Terminal Kinase- and Toll-Like Receptor 4-Dependent and Retinol-Independent Mechanism Molecular and Cellular Biology 2012-05-01 [PMID: 22431523] (Neutralization, Mouse)
• Miao He, Peng Liang IL-24 Transgenic Mice: In Vivo Evidence of Overlapping Functions for IL-20, IL-22, and IL-24 in the Epidermis The Journal of Immunology 2010-02-15 [PMID: 20061404]
• Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S High pressure promotes monocyte adhesion to the vascular wall. Circ. Res., 2007-03-29;100(8):1226-33. 2007-03-29 [PMID: 17395876] (IHC, Mouse)
• Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6. Proc. Natl. Acad. Sci. U.S.A., 2007-02-05;104(7):2319-24. 2007-02-05 [PMID: 17283337] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: Ccl2
• Uniprot:
  • Q5SVU3()