CCL2/JE/MCP-1 Antibody (123616)

Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL2/JE Monoclonal Antibody (Catalog # MAB479). The ND50 is typically 0.75-3.0 µg/mL.

• Reactivity: Mu
• Applications: B/N
• Clone: 123616
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CCL2/JE/MCP-1 Antibody (123616) Summary

• Immunogen: E. coli-derived recombinant mouse CCL2/JE/MCP-1
  Gln24-Arg96
  Accession # P10148
• Specificity: Detects mouse CCL2/JE/MCP-1 in ELISAs. Does not cross-react with recombinant human (rh) CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, rmCCL1, 3, 4, 5, 6, 7, 9/10/MIP-1 gamma , 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or rrCCL20.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Ccl2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.75-3.0 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL2/JE/MCP-1 Antibody (123616)

• C-C motif chemokine ligand 2
• CCL2
• GDCF-2
• HC11
• HSMCR30
• MCAF
• Mcp1
• MCP-1
• SCYA2
• SMC-CF

Background

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The mouse CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

1. Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
2. Gu, L. et al. (1999) Chem. Immunol. 72:7.
3. Luini, W. et al. (1994) Cytokine 6:28.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL2/JE/MCP-1 Antibody (MAB479)(24)

Publications using MAB479

• Namangkalakul, W;Nagai, S;Jin, C;Nakahama, KI;Yoshimoto, Y;Ueha, S;Akiyoshi, K;Matsushima, K;Nakashima, T;Takechi, M;Iseki, S; Augmented effect of fibroblast growth factor 18 in bone morphogenetic protein 2-induced calvarial bone healing by activation of CCL2/CCR2 axis on M2 macrophage polarization Journal of tissue engineering 2023-07-26 [PMID: 37529250] (Neutralization, Mouse)
• X Li, B Yuan, M Lu, Y Wang, N Ding, C Liu, M Gao, Z Yao, S Zhang, Y Zhao, L Xie, Z Chen The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression Nature Communications, 2021-12-10;12(1):7213. 2021-12-10 [PMID: 34893641] (Neutralization, Transgenic Mouse)
• L Wang, J Lan, J Tang, N Luo MCP-1 targeting: Shutting off an engine for tumor development Oncology Letters, 2021-11-19;23(1):26. 11/19/2021 [PMID: 34868363]
• Delphine M. Lees, Louise E. Reynolds, Ana Rita Pedrosa, Marina Roy-Luzarraga, Kairbaan M. Hodivala-Dilke Phosphorylation of pericyte FAK-Y861 affects tumour cell apoptosis and tumour blood vessel regression Angiogenesis 2021-08-01 [PMID: 33730293]
• A Villarreal, MW Tiruneh, J Lee, CF Guerrero-J, J Kuhn, JA David, K Dammeyer, R Mc Kell, J Kwong, PS Rabbani, Q Nie, DJ Ceradini Keratinocyte-Macrophage Crosstalk by the Nrf2/Ccl2/EGF Signaling Axis Orchestrates Tissue Repair Cell Rep, 2020-11-24;33(8):108417. 2020-11-24 [PMID: 33238115] (Western Blot, Mouse)
• L Li, L Ni, RF Heary, S Elkabes Astroglial TLR9 antagonism promotes chemotaxis and alternative activation of macrophages via modulation of astrocyte-derived signals: implications for spinal cord injury J Neuroinflammation, 2020-02-25;17(1):73. 2020-02-25 [PMID: 32098620] (Neutralization, Mouse)
• Sally M. Salah, James D. Meisenheimer, Reena Rao, Jacqueline D. Peda, Darren P. Wallace, Dawson Foster et al. MCP-1 promotes detrimental cardiac physiology, pulmonary edema, and death in the cpk model of polycystic kidney disease American Journal of Physiology-Renal Physiology 2019-08-01 [PMID: 31091126]
• RW Jenkins, AR Aref, PH Lizotte, E Ivanova, S Stinson, CW Zhou, M Bowden, J Deng, H Liu, D Miao, MX He, W Walker, G Zhang, T Tian, C Cheng, Z Wei, S Palakurthi, M Bittinger, H Vitzthum, JW Kim, A Merlino, M Quinn, C Venkataram, JA Kaplan, A Portell, PC Gokhale, B Phillips, A Smart, A Rotem, RE Jones, L Keogh, M Anguiano, L Stapleton, Z Jia, M Barzily-Ro, I Cañadas, TC Thai, MR Hammond, R Vlahos, ES Wang, H Zhang, S Li, GJ Hanna, W Huang, MP Hoang, A Piris, JP Eliane, AO Stemmer-Ra, L Cameron, MJ Su, P Shah, B Izar, M Thakuria, NR LeBoeuf, G Rabinowits, V Gunda, S Parangi, JM Cleary, BC Miller, S Kitajima, R Thummalapa, B Miao, TU Barbie, V Sivathanu, J Wong, WG Richards, R Bueno, CH Yoon, J Miret, M Herlyn, LA Garraway, EM Van Allen, GJ Freeman, PT Kirschmeie, JH Lorch, PA Ott, FS Hodi, KT Flaherty, RD Kamm, GM Boland, KK Wong, D Dornan, CP Paweletz, DA Barbie Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids Cancer Discov, 2017-11-03;0(0):. 2017-11-03 [PMID: 29101162] (Neutralization, Human, Mouse)
• SM Steinberg, T Shabaneh, P Zhang, V Martyanov, Z Li, B Malik, T Wood, A Boni, A Molodtsov, CV Angeles, TJ Curiel, M Whitfield, MJ Turk Myeloid cells that impair immunotherapy are restored in melanomas which acquire resistance to BRAF inhibitors Cancer Res, 2017-02-15;0(0):. 2017-02-15 [PMID: 28202513] (Bioassay, Mouse)
• Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor Nat Med, 2016-05-09;0(0):. 2016-05-09 [PMID: 27158906] (Neutralization, Mouse)
• He Shizhi, He Shuangba, Chen Chun-Hao et al. The chemokine (CCL2-CCR2) signaling axis mediates perineural invasion. Molecular Cancer Research : Mcr 1/1/2015 [PMID: 25312961]
• Kowalewska P, Patrick A, Fox-Robichaud A Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation. PLoS ONE, 2014-09-03;9(9):e104537. 2014-09-03 [PMID: 25184228] (IHC-Fr, Mouse)
• Ellen E Glista-Baker, Alexia J Taylor, Brian C Sayers, Elizabeth A Thompson, James C Bonner Nickel Nanoparticles cause exaggerated lung and airway remodeling in mice lacking the T-box transcription factor, TBX21 (T-bet) Particle and Fibre Toxicology 2014-12-01 [PMID: 24499286]
• Kashiwagi , Satoshi, Yuan , Jianping, Forbes , Benjamin, Hibert , Mathew L, Lee , Eugene L, Whicher , Laura, Goudie , Calum, Yang , Yuan, Chen , Tao, Edelblute , Beth, Collette , Brian, Edington , Laurel, Trussler , James, Nezivar , Jean, Leblanc , Pierre, Bronson , Roderick, Tsukada , Kosuke, Suematsu , Makoto, Dover , Jeffrey, Brauns , Timothy, Gelfand , Jeffrey, Poznansky , Mark C Near-infrared laser adjuvant for influenza vaccine. PLoS ONE, 2013-12-11;8(12):e82899. 2013-12-11 [PMID: 24349390] (IHC, Mouse)
• Scalfone , Lisa K, Nel , Hendrik, Gagliardo , Lucille, Cameron , Jody L, Al-Shokri , Shaikha, Leifer , Cynthia, Fallon , Padraic, Appleton , Judith A Participation of MyD88 and interleukin-33 as innate drivers of Th2 immunity to Trichinella spiralis. Infect Immun, 2013-02-12;81(4):1354-63. 2013-02-12 [PMID: 23403558] (Neutralization, Mouse)
• Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung. J. Immunol., 2012-07-30;189(5):2545-52. 2012-07-30 [PMID: 22851704] (Neutralization, Mouse)
• Uchida Y, Takeshita K, Yamamoto K, Kikuchi R, Nakayama T, Nomura M, Cheng XW, Egashira K, Matsushita T, Nakamura H, Murohara T Stress augments insulin resistance and prothrombotic state: role of visceral adipose-derived monocyte chemoattractant protein-1. Diabetes, 2012-03-06;61(6):1552-61. 2012-03-06 [PMID: 22396205] (In Vivo, Mouse)
• Liu J, Jha P, Lyzogubov VV, Tytarenko RG, Bora NS, Bora PS Relationship between complement membrane attack complex, chemokine (C-C motif) ligand 2 (CCL2) and vascular endothelial growth factor in mouse model of laser-induced choroidal neovascularization. J. Biol. Chem., 2011-04-22;286(23):20991-1001. 2011-04-22 [PMID: 21515678] (Neutralization, Mouse)
• Venet F, Huang X, Chung CS, Chen Y, Ayala A Plasmacytoid dendritic cells control lung inflammation and monocyte recruitment in indirect acute lung injury in mice. Am. J. Pathol., 2009-12-30;176(2):764-73. 2009-12-30 [PMID: 20042672] (Neutralization, Mouse)
• Narasaraju T, Ng HH, Phoon MC MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis. Am. J. Respir. Cell Mol. Biol., 2009-07-17;42(6):732-43. 2009-07-17 [PMID: 19617401] (Neutralization, Mouse)
• Wang T, Dai H, Wan N, Moore Y, Dai Z The role for monocyte chemoattractant protein-1 in the generation and function of memory CD8+ T cells. J. Immunol., 2008-03-01;180(5):2886-93. 2008-03-01 [PMID: 18292510] (Neutralization, Mouse)
• Itaya M, Sakurai E, Nozaki M, Yamada K, Yamasaki S, Asai K, Ogura Y Upregulation of VEGF in murine retina via monocyte recruitment after retinal scatter laser photocoagulation. Invest. Ophthalmol. Vis. Sci., 2007-12-01;48(12):5677-83. 2007-12-01 [PMID: 18055819] (IHC-Fr, Neutralization, Mouse)
• Stamatovic SM, Keep RF, Mostarica-Stojkovic M, Andjelkovic AV CCL2 regulates angiogenesis via activation of Ets-1 transcription factor. J. Immunol., 2006-08-15;177(4):2651-61. 2006-08-15 [PMID: 16888027] (Neutralization, Mouse)
• Penido C, Vieira-de-Abreu A, Bozza MT, Castro-Faria-Neto HC, Bozza PT Role of monocyte chemotactic protein-1/CC chemokine ligand 2 on gamma delta T lymphocyte trafficking during inflammation induced by lipopolysaccharide or Mycobacterium bovis bacille Calmette-Guerin. J. Immunol., 2003-12-15;171(12):6788-94. 2003-12-15 [PMID: 14662884] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: Ccl2
• Uniprot:
  • P10148()