CCL15/MIP-1 delta Antibody [Unconjugated]

363-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/MIP-1d (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL15/MIP-1d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF363). The ND₅₀ is typically 1-5 µg/mL." > 363-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/MIP-1d (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL15/MIP-1d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF363). The ND₅₀ is typically 1-5 µg/mL." title="Recombinant Human CCL15/MIP-1d (363-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/MIP-1d (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL15/MIP-1d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF363). The ND₅₀ is typically 1-5 µg/mL." />

Recombinant Human CCL15/MIP-1d (<a class=

MAB363) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL15/MIP‑1δ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF363) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > MAB363) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL15/MIP‑1δ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF363) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Human CCL15/MIP-1 delta was serially diluted and captured by Mouse Anti-Human CCL15/MIP‑1δ Monoclonal Antibody (Catalog # MAB363) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL15/MIP‑1δ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF363) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Human CCL15/MIP-1 delta was serially diluted and captured by Mouse Anti-Human CCL15/MIP‑1δ Monoclonal Antibody (Catalog # <a class=

• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL15/MIP-1 delta Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL15/MIP-1δ
  Gln22-Ile113
  Accession # Q16663
• Specificity: Detects human CCL15/MIP‑1δ in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL15
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 1-5 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL15/MIP-1 delta Antibody [Unconjugated]

• CCL15
• chemokine (C-C motif) ligand 15
• Chemokine CC-2
• HCC-2
• HCC-2Lkn-1
• Leukotactin 1
• Leukotactin-1
• LKN1
• LKN-1
• Macrophage inflammatory protein 5
• MIP1 delta
• MIP-1 delta
• MIP5
• MIP-5
• MIP-5MIP-1d
• Mrp-2b
• NCC-3
• NCC-3MIP-1D
• NCC3MRP-2B
• new CC chemokine 3
• SCYA15member 15
• Small-inducible cytokine A15

Background

CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma , respectively. The gene for MIP-1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-1δ is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

1. Wang, W. et al. (1998) J. Clinical Immunol. 18:214

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL15/MIP-1 delta Antibody (AF363)(1)

Publication using AF363

• Kominsky SL, Abdelmagid SM, Doucet M, Brady K, Weber KL Macrophage inflammatory protein-1 delta: a novel osteoclast stimulating factor secreted by renal cell carcinoma bone metastasis. Cancer Res., 2008-03-01;68(5):1261-6. 2008-03-01 [PMID: 18316587] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: CCL15
• Uniprot:
  • Q16663()