CCL15/MIP-1 delta Antibody (88119) [Unconjugated]

Recombinant Human CCL15/MIP‑1δ (Catalog # 628-LK) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/MIP‑1δ (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti‑Human CCL15/MIP‑1δ Monoclonal Antibody (Catalog # MAB3631). The ND50 is typically 5-20 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N
• Clone: 88119
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL15/MIP-1 delta Antibody (88119) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL15/MIP-1δ
  Ser46-Ile113
  Accession # Q16663
• Specificity: Detects human CCL15/MIP‑1δ in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL15
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 5-20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL15/MIP-1 delta Antibody (88119) [Unconjugated]

• CCL15
• chemokine (C-C motif) ligand 15
• Chemokine CC-2
• HCC-2
• HCC-2Lkn-1
• Leukotactin 1
• Leukotactin-1
• LKN1
• LKN-1
• Macrophage inflammatory protein 5
• MIP1 delta
• MIP-1 delta
• MIP5
• MIP-5
• MIP-5MIP-1d
• Mrp-2b
• NCC-3
• NCC-3MIP-1D
• NCC3MRP-2B
• new CC chemokine 3
• SCYA15member 15
• Small-inducible cytokine A15

Background

CCL15, also called macrophage inflammatory protein 1 delta (MIP-1δ and Leukotactin-1 (LKN-1), is a CC chemokine with two additional cysteine residues that form a third disulfide bond. CCL15 functions as a chemoattractant for monocytes, T cells, and eosinophils through interactions with CCR1. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma , respectively. The gene for MIP-1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells.

1. Wang, W. et al. (1998) J. Clinical Immunol. 18:214.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCL15
• Uniprot:
  • Q16663()