CCL1/I-309/TCA-3 Antibody (35305) [Unconjugated]

Recombinant Human CCL1/I‑309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I‑309 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL1/I‑309 Monoclonal Antibody (Catalog # MAB272). The ND50 is typically0.1‑0.5 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 35305
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL1/I-309/TCA-3 Antibody (35305) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL1/I‑309/TCA‑3
  Lys24-Lys96
  Accession # P22362
• Specificity: Detects human CCL1/I‑309/TCA‑3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma , 11, 14, 17, 19, 20, 21, 25, 28, recombinant mouse CCL2, 3, 5, 6, 7, 9/10/MIP-1 gamma , 11, 21, or 25 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL1
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.1-0.5 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human CCL1/I-309/TCA-3 Biotinylated Antibody (Catalog number BAF272)
  Standard: Recombinant Human CCL1/I-309/TCA-3 (Catalog number 272-I)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL1/I-309/TCA-3 Antibody (35305) [Unconjugated]

• C-C motif chemokine 1
• CCL1
• chemokine (C-C motif) ligand 1
• I-309
• inflammatory cytokine I-309
• P500
• SCYA1
• SCYA1Small-inducible cytokine A1
• SCYA2
• SISe
• small inducible cytokine A1 (I-309, homologous to mouse Tca-3)
• T lymphocyte-secreted protein I-309
• TCA3
• TCA-3

Background

Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes. CCL1 cDNA encodes a 96 amino acid precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid mature protein. The CCL1 sequence contains one potential N‑linked glycosylation site, and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15‑16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.

1. Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci. USA 89:2950.
2. Miller, M.D. et al. (1990) J. Immunol. 145:2737.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL1/I-309/TCA-3 Antibody (MAB272)(4)

Publications using MAB272

• A Asai, Y Tsuchimoto, H Ohama, H Nishikawa, A Chopra, K Higuchi CD34+CD10+CD19- Cells in Patients with Unhealthy Alcohol Use Stimulate the M2b Monocyte Polarization Cells, 2022-08-30;11(17):. 2022-08-30 [PMID: 36078108] (ELISA Capture, Human)
• A Asai, Y Tsuchimoto, H Ohama, S Fukunishi, Y Tsuda, M Kobayashi, K Higuchi, F Suzuki Host antitumor resistance improved by the macrophage polarization in a chimera model of patients with HCC Oncoimmunology, 2017-03-03;6(4):e1299301. 2017-03-03 [PMID: 28507807] (ICC, Human)
• Montes-Vizuet R, Vega-Miranda A, Valencia-Maqueda E, Negrete-Garcia MC, Velasquez JR, Teran LM CC chemokine ligand 1 is released into the airways of atopic asthmatics. Eur. Respir. J., 2006-03-15;28(1):59-67. 2006-03-15 [PMID: 16540498] (ELISA Development, Neutralization, Human)
• Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases. J. Leukoc. Biol., 2001-10-01;70(4):617-23. 2001-10-01 [PMID: 11590199] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: CCL1
• Uniprot:
  • P22362()