CCDC49 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: CCDC49 Antibody [NBP2-49381] - Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: CCDC49 Antibody [NBP2-49381] - Staining in human bone marrow and liver tissues using anti-CWC25 antibody. Corresponding CWC25 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CCDC49 Antibody [NBP2-49381] - Staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.
Immunohistochemistry-Paraffin: CCDC49 Antibody [NBP2-49381] - Staining of human bone marrow shows high expression.
Immunohistochemistry-Paraffin: CCDC49 Antibody [NBP2-49381] - Staining of human liver shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CCDC49 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RHAPGYTRKLSAEELERKRQEMMENAKWREEERLNILKRHAKDEEREQRLEKLDSRDGKFIHRMKLESASTSSLEDRVKRNIYSLQRTSVALEKNF
Specificity
Specificity of human CCDC49 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (90%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CWC25
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CCDC49 Recombinant Protein Antigen (NBP2-49381PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CCDC49 Antibody

  • CCDC49
  • coiled-coil domain containing 49
  • Coiled-coil domain-containing protein 49
  • CWC25 spliceosome-associated protein homolog (S. cerevisiae)
  • DKFZp779M0968
  • FLJ20291
  • pre-mRNA-splicing factor CWC25 homolog
  • Spliceosome-associated protein homolog CWC25

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CWC25