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GPKOW Antibody

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Western Blot: GPKOW Antibody [NBP1-81800] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GPKOW antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Genetic Strategies: Western Blot: GPKOW Antibody [NBP1-81800] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GPKOW antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: GPKOW Antibody [NBP1-81800] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81800] - Staining of human fallopian tube shows nuclear positivity in glandular cells.
Western Blot: GPKOW Antibody [NBP1-81800] - Analysis in human cell line A-549.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

    

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GPKOW Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MPRPDEEQEKDKEDQPQGLVPGGAVVVLSGPHRGLYGKVEGLDPDNVRAMVRLAVGSRVVTVSEYYLRPVSQQEFDKNTLDLRQQNGTASSRKTLWNQELYIQQDNSERKRKHLPD
Marker
Nucleus Marker
Specificity
Specificity of human GPKOW antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPKOW
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
GPKOW Protein (NBP1-81800PEP)
Publications
Read Publication using NBP1-81800.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GPKOW Antibody

  • G patch domain and KOW motifs
  • GPATC5G patch domain and KOW motifs-containing protein
  • GPATCH5G patch domain-containing protein 5
  • Protein MOS2 homolog
  • Spp2
  • T54Protein T54

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP), immobilized onto a microplate, supports the adhesion of the HEK293 human embryonic kidney cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Osteopontin/OPN (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808). The ND50 is typically 1‑3 µg/mL.
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Sphingosine 1
phosphate phosphatase ...
NBP1-53181
Western Blot: Sphingosine 1 phosphate phosphatase 2 Antibody [NBP1-53181] - Titration: 2.5ug/ml Positive Control: HepG2 cell lysate.
Immunohistochemistry-Paraffin: Sphingosine 1 phosphate phosphatase 2 Antibody [NBP1-53181] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Species: Hu
Applications: WB, IHC, IHC-P
CXXC1 Antibody
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Western Blot: CXXC1 Antibody [NB100-56630] - analysis at 1:1,000 dilution against 15 ug/lane of human spleen lysate. Immunoreactivity was determined using SuperSignal system (Pierce) and by exposing to the film for 15 minutes.
(1 Publication)
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DHX16 Antibody
NBP2-13920
Immunocytochemistry/Immunofluorescence: DHX16 Antibody [NBP2-13920] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: DHX16 Antibody [NBP2-13920] - Staining in human testis and liver tissues using anti-DHX16 antibody. Corresponding DHX16 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: DHX16 Antibody [NBP2-13920] - Staining of human kidney shows strong nuclear positivity in renal tubules and glomeruli.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Chk2 Antibody
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NB100-56546
Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Analysis using Azide Free version of NB100-56546. Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) [NB100-56546] - Analysis using Azide Free version of NB100-56546. Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - analysis of Chk2 in A) human HEK293 and B) mouse NIH3T3 lysate using Chk2 antibody at 2 ug/ml.
Species: Hu, Mu
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TMEM171 Antibody
NBP1-80876
Western Blot: TMEM171 Antibody [NBP1-80876] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: TMEM171 Antibody [NBP1-80876] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Species: Hu
Applications: WB, IHC, IHC-P
DHX38 Antibody
NBP1-85269
Western Blot: DHX38 Antibody [NBP1-85269] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/Immunofluorescence: DHX38 Antibody [NBP1-85269] - Staining of human cell line U-2 OS shows positivity in nucleus.
Immunohistochemistry-Paraffin: DHX38 Antibody [NBP1-85269] - Staining of human esophagus shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
SLU7 Antibody
NBP2-20403
Western Blot: SLU7 Antibody [NBP2-20403] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates, B: hSLU7 transfected 293T lysates 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: SLU7 Antibody [NBP2-20403] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.
Western Blot: SLU7 Antibody [NBP2-20403] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.
Species: Hu, Mu
Applications: WB, IHC, IHC-P
CDC2/CDK1 Antibody
NBP1-85729
Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues. Corresponding CDK1 RNA-seq data are presented for the same tissues.
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DHX8 Antibody
NBP2-13923
Western Blot: DHX8 Antibody [NBP2-13923] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/Immunofluorescence: DHX8 Antibody [NBP2-13923] - Staining of human cell line HEK 293 shows positivity in nucleus but not nucleoli.
Immunohistochemistry-Paraffin: DHX8 Antibody [NBP2-13923] - Staining of human cerebellum shows strong cytoplasmic and nucleolar positivity in Purkinje cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Chk1 Antibody
(2G1D5)
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Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Western blot analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).
Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2G1D5) [NB100-1768] - Chk1 antibody was tested at 1:10 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) [NB100-1768] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human lung using 1:250 conc. of Chk1 antibody (clone 2G1D5) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and nuclear staining of Chk1 was observed as this is a ubiquitous cell checkpoint protein. Staining was performed by Histowiz.
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Applications: WB, ICC/IF, IHC, IHC-P
DNA Polymerase
delta, catalytic subu ...
NBP2-33466
Western Blot: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
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(2 Publications)
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NBP2-13303
Western Blot: SGPP1 Antibody [NBP2-13303] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/Immunofluorescence: SGPP1 Antibody [NBP2-13303] - Staining of human cell line A-431 shows localization to nucleoplasm.
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NBP1-87267
Western Blot: PRKX Antibody [NBP1-87267] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4
Immunocytochemistry/Immunofluorescence: PRKX Antibody [NBP1-87267] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus.
Immunohistochemistry-Paraffin: PRKX Antibody [NBP1-87267] - Staining of human small intestine shows strong nuclear positivity in glandular cells.
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NBP2-22273
Western Blot: PRPF8 Antibody [NBP2-22273] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22273 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: PRPF8 Antibody [NBP2-22273] - Samples: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti- PRPF8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: PRPF8 Antibody [NBP2-22273] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody NBP2-22273 used for IP at 6 ug/mg lysate. PRPF8 was also immunoprecipitated by rabbit anti-PRPF8 antibodies NBP2-22272 and NBP2-22274 For blotting immunoprecipitated PRPF8, NBP2-22273 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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BMP-2
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355-BM/CF
BMP-2 [Unconjugated]
(116 Publications)
Species: Hu, Mu, Rt
GPKOW Antibody
NBP1-81800
GPKOW Antibody
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD

Publications for GPKOW Antibody (NBP1-81800)(1)


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Bioinformatics

Gene Symbol GPKOW