CC2D1A Antibody - BSA Free

Western Blot: CC2D1A Antibody [NBP3-12658] - Lane A: Hela Whole Cell LysateLane B: HEK-293 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:130 kDa

Immunohistochemistry-Paraffin: CC2D1A Antibody [NBP3-12658] - Staining of human CC2D1A in human liver with rabbit polyclonal antibody at 1:100 dilution.

Immunoprecipitation: CC2D1A Antibody [NBP3-12658] - Lane A:0.5 mg MCF7 Whole Cell Lysate4 uL anti-CC2D1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CC2D1A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 104 kDaObserved band size :130 kDa

Immunocytochemistry/ Immunofluorescence: CC2D1A Antibody - BSA Free [NBP3-12658] - Immunofluorescence staining of CC2D1A in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12658 (dilution ratio 1:200) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CC2D1A Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human CC2D1A fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CC2D1A
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of uLysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for CC2D1A Antibody - BSA Free

• Akt kinase-interacting protein 1
• coiled-coil and C2 domain containing 1A
• coiled-coil and C2 domain-containing protein 1A
• Five repressor element under dual repression-binding protein 1
• FLJ20241
• FLJ41160
• FRE under dual repression-binding protein 1
• FREUD-1
• mental retardation, nonsyndromic, autosomal recessive, 3
• MRT3Freud-1/Aki1
• Putative NF-kappa-B-activating protein 023N
• putative NFkB activating protein

Background

The CC2D1A gene encodes a transcriptional repressor that binds to a conserved 14-bp 5'-repressor element and regulates expression of the 5-hydroxytryptamine (serotonin) receptor 1A gene in neuronal cells. The DNA binding and transcriptional repressor activities

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CC2D1A