![Immunocytochemistry/Immunofluorescence: CBX1 Antibody (RAB-C145) - Chimeric - Azide and BSA Free [NBP3-20129] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-C145 (NBP3-20129) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-20129, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20129_rabbit-cbx1-mab-rab-c145-chimeric-azide-and-bsa-free-2742023924272.png "Immunocytochemistry/Immunofluorescence: CBX1 Antibody (RAB-C145) - Chimeric - Azide and BSA Free [NBP3-20129] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-C145 (NBP3-20129) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-20129, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IP, ChIP, MS |
| Clone | RAB-C145 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit CBX1 Antibody (RAB-C145) - Chimeric - Azide and BSA Free (NBP3-20129) is a recombinant monoclonal antibody validated for use in WB, ELISA, Flow, ICC/IF, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained by recombinant antibody (rAb) phage display recognizing CBX1 protein under non-denaturing conditions. |
| Epitope | This antibody binds to a folded domain, amino acids 20-73. |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CBX1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CBX1 Antibody (NBP3-20129)Find related products by research area.
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| Gene Symbol | CBX1 |
![Western Blot: CBX1 Antibody (RAB-C145) - Chimeric - Azide and BSA Free [NBP3-20129] - Nuclear lysates of HeLa(A) (0.3ug/ml), HEK293(B) (0.3ug/ml) and MCF7(C) (0.1ug/ml) (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C145 (NBP3-20129) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20129_rabbit-cbx1-mab-rab-c145-chimeric-azide-and-bsa-free-2742023924257.png "Western Blot: CBX1 Antibody (RAB-C145) - Chimeric - Azide and BSA Free [NBP3-20129] - Nuclear lysates of HeLa(A) (0.3ug/ml), HEK293(B) (0.3ug/ml) and MCF7(C) (0.1ug/ml) (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C145 (NBP3-20129) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: CBX1 Antibody (RAB-C145) - Chimeric - Azide and BSA Free [NBP3-20129] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C145 (NBP3-20129, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20129_rabbit-cbx1-mab-rab-c145-chimeric-azide-and-bsa-free-274202392429.png "Flow Cytometry: CBX1 Antibody (RAB-C145) - Chimeric - Azide and BSA Free [NBP3-20129] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C145 (NBP3-20129, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
