CBLB Antibody

Western Blot: CBLB Antibody [NBP1-86599] - Analysis in control (vector only transfected HEK293T lysate) and CBLB over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: CBLB Antibody [NBP1-86599] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-86599] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-86599] - Staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells.

Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-86599] - Staining of human endometrium shows strong granular cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CBLB Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DNRLSRHIHHVESVPSRDPPMPLEAWCPRDVFGTNQLVGCRLLGEGSPKPGITASSNVNGRHSRVGSDPVLMRKHRRHDLPLEGAKVFSNGHLGSEEYDVPPRLSPPPPVTTLLPSIKCTGPLA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CBLB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CBLB Protein (NBP1-86599PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (84%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CBLB Antibody

• Cas-Br-M (murine) ecotropic retroviral transforming sequence b
• Cas-Br-M (murine) ectropic retroviral transforming sequence b
• Casitas B-lineage lymphoma proto-oncogene b
• CBLB
• Cbl-B
• DKFZp686J10223
• DKFZp779A0729
• DKFZp779F1443
• E3 ubiquitin-protein ligase CBL-B
• EC 6.3.2
• EC 6.3.2.-
• FLJ41152
• Nbla00127
• RING finger protein 56
• RNF56
• RNF56FLJ36865
• SH3-binding protein CBL-B
• Signal transduction protein CBL-B

Background

CBLB - Cas-Br-M (murine) ecotropic retroviral transforming sequence b

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CBLB