CBLB Antibody - BSA Free

Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-49919] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Cbl-b used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: CBLB Antibody [NBP1-49919] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cbl-b antibody NBP1-49919 used for IP at 3 ug/mg lysate. Cbl-b was also immunoprecipitated by rabbit anti-Cbl-b antibody NBP1-49920, which recognizes a downstream epitope. For blotting immunoprecipitated Cbl-b, NBP1-49920 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu, Rt
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CBLB Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CBLB Antibody - BSA Free (NBP1-49919) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 450 and 500 of human Cas-Br-M (murine) Ecotropic Retroviral Transforming Sequence b using the numbering given in entry NP_733762.2 (GeneID 868).
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CBLB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CBLB Antibody - BSA Free

• Cas-Br-M (murine) ecotropic retroviral transforming sequence b
• Cas-Br-M (murine) ectropic retroviral transforming sequence b
• Casitas B-lineage lymphoma proto-oncogene b
• CBLB
• Cbl-B
• DKFZp686J10223
• DKFZp779A0729
• DKFZp779F1443
• E3 ubiquitin-protein ligase CBL-B
• EC 6.3.2
• EC 6.3.2.-
• FLJ41152
• Nbla00127
• RING finger protein 56
• RNF56
• RNF56FLJ36865
• SH3-binding protein CBL-B
• Signal transduction protein CBL-B

Background

CBLB is an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CBLB Antibody (NBP1-49919). (Showing 1 - 1 of 1 FAQ).

1. One of our customers bought one vial of NBP1-49919, and he told me the website said it can be used in WB, but when he got the antibody, he find the website said as follows, the customer is a little confused about the WB (-). Does this mean that it can't be used in WB? Please give us some help and thanks.
   • Regarding your customer's inquiry, our CBLB antibody (NBP1-49919) has been validated for IHC and it is not recommended to use this product for western blot. The negative mark (-) indicates this product should not be used for western blot. I am sorry if this has caused confusion for your customers.

Bioinformatics

• Gene Symbol: CBLB