CBL Antibody

Western Blot: CBL Antibody [NBP1-28758] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-c-Cbl antibody NBP1-28758 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. c-Cbl was also immunoprecipitated by rabbit anti-c-Cbl antibody NBP1-28757, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CBL Antibody Summary

• Description: Novus Biologicals Rabbit CBL Antibody (NBP1-28758) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 675 and 725 of human Casitas B-lineage lymphoma proto-oncogene using the numbering given in entry NP_005179.2 (GeneID 867).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CBL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CBL Antibody

• Cas-Br-M (murine) ecotropic retroviral transforming sequence
• Casitas B-lineage lymphoma proto-oncogene
• CBL
• CBL2
• CBL2E3 ubiquitin-protein ligase CBL
• EC 6.3.2
• EC 6.3.2.-
• oncogene CBL2
• Proto-oncogene c-Cbl
• RING finger protein 55
• RNF55
• RNF55C-CBL
• Signal transduction protein CBL

Background

The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CBL
• Entrez:
  • 867(Human)
• Uniprot:
  • NP_005179.2(Human)