CBARA1 Antibody - BSA Free

Immunohistochemistry-Paraffin: CBARA1 Antibody [NBP1-86663] - Staining of human liver shows moderate granular positivity in cytoplasm in hepatocytes.

Immunohistochemistry-Paraffin: CBARA1 Antibody [NBP1-86663] - Staining of human endometrium shows strong granular positivity in cytoplasm in glandular cells.

Immunohistochemistry-Paraffin: CBARA1 Antibody [NBP1-86663] - Staining of human kidney shows strong granular positivity in cytoplasm in cells in tubules.

Immunohistochemistry-Paraffin: CBARA1 Antibody [NBP1-86663] - Staining of human rectum shows strong granular positivity in cytoplasm in glandular cells.

Simple Western: CBARA1 Antibody [NBP1-86663] - Electropherogram image(s) of corresponding Simple Western lane view. CBARA1 antibody was used at 1:20 dilution on RT-4 and U-251 lysate(s).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CBARA1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRITERQFGGMLLAYSGVQSKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFL
• Predicted Species: Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MICU1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Simple Western 1:20
  • Western Blot Reported in scientific literature (PMID 28292968)
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. See Simple Western Antibody Database for Simple Western validation: Tested in RT-4, U-251, separated by Size, antibody dilution of 1:20, apparent MW was 52 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  CBARA1 Protein (NBP1-86663PEP)

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32075766).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CBARA1 Antibody - BSA Free

• allergen Hom s 4
• ara CALC
• Atopy-related autoantigen CALC
• CALC
• CALCDKFZp564C246
• calcium binding atopy-related autoantigen 1
• Calcium-binding atopy-related autoantigen 1
• CBARA1
• MICU1
• MICU1mitochondrial
• mitochondrial calcium uptake 1

Background

CBARA1 - calcium binding atopy-related autoantigen 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CBARA1 Antibody (NBP1-86663)(6)

Publications using NBP1-86663

• Di Marco G, Vallese F, Jourde B et al. A High-Throughput Screening Identifies MICU1 Targeting Compounds Cell Rep 2020-02-18 [PMID: 32075766] (WB, Mouse)
• Ji L, Liu F, Jing Z et al. MICU1 Alleviates Diabetic Cardiomyopathy Through Mitochondrial Ca(2+)-dependent Antioxidant Response. Diabetes. 2017-03-14 [PMID: 28292968] (WB, IHC-P)
• Lee Y, Min CK, Kim TG et al. Structure and function of the N-terminal domain of the human mitochondrial calcium uniporter. EMBO Rep 2015-10-01 [PMID: 26341627] (WB, Human)
• Wang W, Xie Q, Zhou X et al. Mitofusin-2 triggers mitochondria Ca(2+) influx from the endoplasmic reticulum to induce apoptosis in hepatocellular carcinoma cells. Cancer Lett. 2014-12-23 [PMID: 25541060] (WB, IHC-P, Human)
  
  Details:
  CBARA1 (MICU1) antibody used for immunohistochemisty on paraffin-embedded HCC and adjacent normal tissues to compare the expression of CBARA1 (MICU1). Immunohistochemistry analysis showing no significant difference between HCC and adjacent normal tissues. Additionally, western blot analysis showing low protein expression levels of MICU1 (Fig. 11).
• Stephanie S Lam, Jeffrey D Martell, Kimberli J Kamer et al. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nature Methods 2014-11-24 [PMID: 25419960] (WB, Human)
• Logan CV, Szabadkai G, Sharpe JA et al. Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling. Nat Genet 2014-02-01 [PMID: 24336167]

Review for CBARA1 Antibody (NBP1-86663) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Prabir Chakraborty WB Human 06/11/2014

Summary

• Application: Western Blot
• Sample Tested: HUMAN CANCER CELL LINES, WHOLE CELL LYSATES
• Species: Human
• Lot: B90039

Bioinformatics

• Gene Symbol: MICU1
• Entrez:
  • 10367(Human)
• Uniprot:
  • Q9BPX6(Human)