Cathepsin L/V/K/H Antibody (JM10-78)

Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Western blot analysis of Cathepsin L/V/K/H on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate

Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded rat kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.

Flow Cytometry: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of HepG2 cells with Cathepsin L/V/K/H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of Cathepsin L/V/K/H on different cells lysates using anti-Cathepsin L/V/K/H antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: A549

Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded human kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded mouse kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: JM10-78
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Cathepsin L/V/K/H Antibody (JM10-78) Summary

• Description: Novus Biologicals Rabbit Cathepsin L/V/K/H Antibody (JM10-78) (NBP2-67216) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-Cathepsin L/V/K/H Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Cathepsin L/V/K/H aa 92-138 / 333. (SwissProt: P07711 Human; SwissProt: O60911 Human; SwissProt: P43235 Human; SwissProt: P09668 Human)
• Localization: Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane, Nucleus, Secreted.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CTSL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cathepsin L/V/K/H Antibody (JM10-78)

• ACC4
• ACC-4
• ACC5
• ACC-5
• aleurain
• cathepsin B3
• cathepsin BA
• cathepsin K
• cathepsin L1
• cathepsin L2
• cathepsin L2, preproprotein
• Cathepsin O
• cathepsin O1
• Cathepsin O2
• Cathepsin U
• Cathepsin X
• CATL
• CATL2
• CPSB
• CTS02
• CTSL1
• CTSL2
• CTSO
• CTSO1
• CTSO2
• CTSU
• Major excreted protein
• MEP
• N-benzoylarginine-beta-naphthylamide hydrolase
• PKND
• procathepsin L
• PYCD

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin L/V/K/H Antibody (NBP2-67216)(1)

Publication using NBP2-67216

• Xie YX, Naseri NN, Fels J et al. Lysosomal exocytosis releases pathogenic alpha-synuclein species from neurons in synucleinopathy models Nature communications 2022-08-22 [PMID: 35995799] (WB, Mouse)

Bioinformatics

• Gene Symbol: CTSL