Cathepsin L Antibody (33/2)

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Western Blot: Cathepsin L Antibody (33/2) [NB100-1775] - Whole cell extract of Mv 1 Lu cultured mink lung was separated on SDS-PAGE and blotted with Monoclonal Anti-Cathepsin L. The antibody was developed with Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, MdSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, MiAr
Clone
33/2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Cathepsin L Antibody (33/2) Summary

Immunogen
Purified full length native protein (Human lung cancer line EPLC 32 M1).
Epitope
Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule).
Localization
Lysosomes
Specificity
Reacts specifically with native and denatured forms of human cathepsin L and procathepsin L.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CTSL1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Microarray
Application Notes
The antibody may be used for the suppression of the malignant growth of myeloma cells. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Positive Control
Cathepsin L Lysate (NBL1-09594)
Publications
Read Publications using
NB100-1775 in the following applications:

Reactivity Notes

Cross-reacts with Mouse procathepsin L (weak), Rat cathepsin L (strong), and Mink cathepsin and procathepsin L. Does not cross-react with human cathepsin types B, D, H and S, procathepsins B and D, rat cathepsin B, and mouse procathepsins B and D. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Cathepsin L Antibody (33/2)

  • Cathepsin L
  • cathepsin L1
  • CATL
  • CTSL
  • CTSL1
  • CTSLEC 3.4.22.15
  • EC 3.4.22
  • FLJ31037
  • Major excreted protein
  • MEP

Background

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumour invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intralysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive proform, and mRNA expression level seems to correlate with their metastatic potential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin L Antibody (NB100-1775)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cathepsin L Antibody (NB100-1775) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CTSL1
Entrez
Uniprot