Western blot shows 100 ng of Recombinant Human Cathepsin L (Catalog # 952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin ...read more
Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 5 µg/mL overnight at 4 °C. Tissue was stained ...read more
Cathepsin L was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 8 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
Cathepsin L Antibody (204101) [Unconjugated] Summary
Mouse myeloma cell line NS0-derived recombinant human Cathepsin L Glu113-Val333 Accession # P07711
Detects human and mouse Cathepsin L in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin B, C, L2, O, S, or X/Z/P is observed. In Western blots, both the pro and active forms of recombinant human and mouse Cathspsin L are recognized.
Protein A or G purified from hybridoma culture supernatant
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Cathepsin L Antibody (204101) [Unconjugated]
Major excreted protein
is a lysosomal cysteine protease expressed in most eukaryotic cells.
Cathepsin L is known to hydrolyze a number of proteins, including the
proform of urokinase-type plasminogen activator, which is activated by
Cathepsin L cleavage (1). Cathepsin L has also been shown to
proteolytically inactivate alpha 1-antitrypsin and secretory
leucoprotease inhibitor, two major protease inhibitors of the
respiratory tract (2). These observations, combined with the
demonstration of increased Cathepsin L activity in the epithelial lining
fluid of the lungs of emphysema patients, have led to the suggestion
that the enzyme may be involved in the progression of this disease.
Cathepsin L has also been identified as a major excreted protein of
transformed fibroblasts, indicating the enzyme could be involved in
malignant tumor growth (3).
Goretzki, L. et al. (1992) FEBS Lett. 297:112.
Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.