Cathepsin L Antibody (204101) [Unconjugated]

952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. For natural samples, we recommend the use of Catalog # AF1515." > 952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. For natural samples, we recommend the use of Catalog # AF1515." title="Western blot shows 100 ng of Recombinant Human Cathepsin L (Catalog # 952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. For natural samples, we recommend the use of Catalog # AF1515." />

Western blot shows 100 ng of Recombinant Human Cathepsin L (Catalog # <a class=

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." > CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." title="Cathepsin L was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." />

Cathepsin L was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clone: 204101
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Cathepsin L Antibody (204101) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cathepsin L
  Glu113-Val333
  Accession # P07711
• Specificity: Detects human and mouse Cathepsin L in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin B, C, L2, O, S, or X/Z/P is observed. In Western blots, both the pro and active forms of recombinant human and mouse Cathspsin L are recognized.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CTSL
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin L Antibody (204101) [Unconjugated]

• Cathepsin L
• cathepsin L1
• CATL
• CTSL
• CTSL1
• CTSLEC 3.4.22.15
• EC 3.4.22
• FLJ31037
• Major excreted protein
• MEP

Background

Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage (1). Cathepsin L has also been shown to proteolytically inactivate alpha 1-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract (2). These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth (3).

1. Goretzki, L. et al. (1992) FEBS Lett. 297:112.
2. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
3. Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin L Antibody (MAB9521)(13)

Publications using MAB9521

• Teneale A. Stewart, Katherine Hughes, Alexander J. Stevenson, Natascia Marino, Adler L. Ju, Michael Morehead et al. Mammary mechanobiology - investigating roles for mechanically activated ion channels in lactation and involution Journal of Cell Science 2020-05-12 [PMID: 33262312] (Western Blot, Mouse)
• Y Li, JW Jones, H M C Choi, C Sarkar, MA Kane, EY Koh, MM Lipinski, J Wu cPLA2 activation contributes to lysosomal defects leading to impairment of autophagy after spinal cord injury Cell Death Dis, 2019-07-11;10(7):531. 7/11/2019 [PMID: 31296844]
• C Sarkar, JW Jones, N Hegdekar, JA Thayer, A Kumar, AI Faden, MA Kane, MM Lipinski PLA2G4A/cPLA2-mediated lysosomal membrane damage leads to inhibition of autophagy and neurodegeneration after brain trauma Autophagy, 2019-06-25;0(0):1-20. 2019-06-25 [PMID: 31238788] (IHC, Mouse)
• CCT complex restricts neuropathogenic protein aggregation via autophagy Nat Commun, 2016-12-08;7(0):13821. 2016-12-08 [PMID: 27929117] (Western Blot, Human)
• A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy Sci Adv, 2016-09-02;2(9):e1601167. 2016-09-02 [PMID: 27617292] (Western Blot, Mouse)
• Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo. Cell Death Differ, 2014-06-06;21(9):1442-50. 2014-06-06 [PMID: 24902901] (IHC-P, Western Blot, Mouse)
• Jonathan J. Campbell, Laur-Alexandru Botos, Timothy J. Sargeant, Natalia Davidenko, Ruth E. Cameron, Christine J. Watson A 3-D in vitro co-culture model of mammary gland involution Integr Biol (Camb) 2014-04-12 [PMID: 24722402]
• O'Brien J, Martinson H, Durand-Rougely C Macrophages are crucial for epithelial cell death and adipocyte repopulation during mammary gland involution. Development, 2011-11-30;139(2):269-75. 2011-11-30 [PMID: 22129827] (Western Blot, Mouse)
• Kreuzaler PA, Staniszewska AD, Li W, Omidvar N, Kedjouar B, Turkson J, Poli V, Flavell RA, Clarkson RW, Watson CJ Stat3 controls lysosomal-mediated cell death in vivo. Nat. Cell Biol., 2011-02-20;13(3):303-9. 2011-02-20 [PMID: 21336304] (Western Blot, Mouse)
• Lopez-Castejon G, Theaker J, Pelegrin P P2X(7) receptor-mediated release of cathepsins from macrophages is a cytokine-independent mechanism potentially involved in joint diseases. J. Immunol., 2010-07-16;185(4):2611-9. 2010-07-16 [PMID: 20639492] (Western Blot, Human, Mouse)
• Zeeuwen PL, van Vlijmen-Willems IM, Cheng T The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea. FASEB J., 2010-05-21;24(10):3744-55. 2010-05-21 [PMID: 20495178] (IHC-P, Mouse)
• Parker BS, Ciocca DR, Bidwell BN, Gago FE, Fanelli MA, George J, Slavin JL, Moller A, Steel R, Pouliot N, Eckhardt BL, Henderson MA, Anderson RL Primary tumour expression of the cysteine cathepsin inhibitor Stefin A inhibits distant metastasis in breast cancer. J. Pathol., 2008-02-01;214(3):337-46. 2008-02-01 [PMID: 17985332] (IHC-P, Mouse)
• Cheng T, Hitomi K, van Vlijmen-Willems IM, de Jongh GJ, Yamamoto K, Nishi K, Watts C, Reinheckel T, Schalkwijk J, Zeeuwen PL Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification. J. Biol. Chem., 2006-03-24;281(23):15893-9. 2006-03-24 [PMID: 16565075] (IHC, Human)

Bioinformatics

• Gene Symbol: CTSL
• Uniprot:
  • P07711()