Cathepsin L Antibody (204101) [Unconjugated]

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Western blot shows 100 ng of Recombinant Human Cathepsin L (Catalog # 952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin ...read more
Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 5 µg/mL overnight at 4 °C. Tissue was stained ...read more
Cathepsin L was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 8 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clone
204101
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

Cathepsin L Antibody (204101) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin L
Glu113-Val333
Accession # P07711
Specificity
Detects human and mouse Cathepsin L in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin B, C, L2, O, S, or X/Z/P is observed. In Western blots, both the pro and active forms of recombinant human and mouse Cathspsin L are recognized.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CTSL1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Immunoprecipitation 25 ug/mL
Publications
Read Publications using
MAB9521 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin L Antibody (204101) [Unconjugated]

  • Cathepsin L
  • cathepsin L1
  • CATL
  • CTSL
  • CTSL1
  • CTSLEC 3.4.22.15
  • EC 3.4.22
  • FLJ31037
  • Major excreted protein
  • MEP

Background

Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage (1). Cathepsin L has also been shown to proteolytically inactivate alpha 1-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract (2). These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth (3).
  1. Goretzki, L. et al. (1992) FEBS Lett. 297:112.
  2. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
  3. Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin L Antibody (MAB9521)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-P, Western Blot.


Filter By Application
IHC
(1)
IHC-P
(2)
Western Blot
(6)
All Applications
Filter By Species
Human
(3)
Mouse
(6)
All Species
Showing Publications 1 - 9 of 9.
Publications using MAB9521 Applications Species
CCT complex restricts neuropathogenic protein aggregation via autophagy Nat Commun, 2016;7(0):13821. 2016 [PMID: 27929117] (Western Blot, Human) Western Blot Human
A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy Sci Adv, 2016;2(9):e1601167. 2016 [PMID: 27617292] (Western Blot, Mouse) Western Blot Mouse
Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo. Cell Death Differ, 2014;21(9):1442-50. 2014 [PMID: 24902901] (Western Blot, Mouse) Western Blot Mouse
O'Brien J, Martinson H, Durand-Rougely C Macrophages are crucial for epithelial cell death and adipocyte repopulation during mammary gland involution. Development, 2012;139(2):269-75. 2012 [PMID: 22129827] (Western Blot, Mouse) Western Blot Mouse
Kreuzaler PA, Staniszewska AD, Li W, Omidvar N, Kedjouar B, Turkson J, Poli V, Flavell RA, Clarkson RW, Watson CJ Stat3 controls lysosomal-mediated cell death in vivo. Nat. Cell Biol., 2011;13(3):303-9. 2011 [PMID: 21336304] (Western Blot, Mouse) Western Blot Mouse
Lopez-Castejon G, Theaker J, Pelegrin P P2X(7) receptor-mediated release of cathepsins from macrophages is a cytokine-independent mechanism potentially involved in joint diseases. J. Immunol., 2010;185(4):2611-9. 2010 [PMID: 20639492] (Western Blot, Human) Western Blot Human
Zeeuwen PL, van Vlijmen-Willems IM, Cheng T The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea. FASEB J., 2010;24(10):3744-55. 2010 [PMID: 20495178] (IHC-P, Mouse) IHC-P Mouse
Parker BS, Ciocca DR, Bidwell BN, Gago FE, Fanelli MA, George J, Slavin JL, Moller A, Steel R, Pouliot N, Eckhardt BL, Henderson MA, Anderson RL Primary tumour expression of the cysteine cathepsin inhibitor Stefin A inhibits distant metastasis in breast cancer. J. Pathol., 2008;214(3):337-46. 2008 [PMID: 17985332] (IHC-P, Mouse) IHC-P Mouse
Cheng T, Hitomi K, van Vlijmen-Willems IM, de Jongh GJ, Yamamoto K, Nishi K, Watts C, Reinheckel T, Schalkwijk J, Zeeuwen PL Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification. J. Biol. Chem., 2006;281(23):15893-9. 2006 [PMID: 16565075] (IHC, Human) IHC Human

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Bioinformatics

Gene Symbol CTSL1
Uniprot
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