Western blot shows 100 ng of Recombinant Human Cathepsin L (Catalog # 952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin ...read more
Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 5 µg/mL overnight at 4 °C. Tissue was stained ...read more
Cathepsin L was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 8 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
Cathepsin L Antibody (204101) [Unconjugated] Summary
Mouse myeloma cell line NS0-derived recombinant human Cathepsin L Glu113-Val333 Accession # P07711
Detects human and mouse Cathepsin L in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin B, C, L2, O, S, or X/Z/P is observed. In Western blots, both the pro and active forms of recombinant human and mouse Cathspsin L are recognized.
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Cathepsin L Antibody (204101) [Unconjugated]
Major excreted protein
is a lysosomal cysteine protease expressed in most eukaryotic cells.
Cathepsin L is known to hydrolyze a number of proteins, including the
proform of urokinase-type plasminogen activator, which is activated by
Cathepsin L cleavage (1). Cathepsin L has also been shown to
proteolytically inactivate alpha 1-antitrypsin and secretory
leucoprotease inhibitor, two major protease inhibitors of the
respiratory tract (2). These observations, combined with the
demonstration of increased Cathepsin L activity in the epithelial lining
fluid of the lungs of emphysema patients, have led to the suggestion
that the enzyme may be involved in the progression of this disease.
Cathepsin L has also been identified as a major excreted protein of
transformed fibroblasts, indicating the enzyme could be involved in
malignant tumor growth (3).
Goretzki, L. et al. (1992) FEBS Lett. 297:112.
Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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