Cathepsin K Assay Kit (Fluorometric)

Cathepsin K Assay Kit (Fluorometric) [NBP2-54842] - Cathepsin K Activity measured in tissue lysates (each with protein concentration of 4 mg/mL) after 1 hour of incubation with and without inhibitor (I).

Cathepsin K Assay Kit (Fluorometric) [NBP2-54842] - Cathepsin K Activity measured in cell lysates (each with cells concentration of 4e7 cells/mL) after 1 hour of incubation with and without inhibitor (I).

Product Discontinued

Cathepsin K Assay Kit (Fluorometric) Summary

• Description: Rapid and convenient fluorometric assay to measure Cathepsin K Activity within 1-2 hrs.
  
  Detection method: Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex/Em = 400/505 nm)Applications: Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can intiate or propagate proapaptotic signals.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65303)
• Kit Type: Assay Kit (Fluorometric)
• Gene: CTSK

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Cathepsin K Assay Kit (Fluorometric)

• cathepsin K (pycnodysostosis)
• Cathepsin K
• Cathepsin O
• cathepsin O1
• Cathepsin O2
• Cathepsin X
• CTS02
• CTSK
• CTSO
• CTSO1
• CTSO2
• EC 3.4.22
• EC 3.4.22.38
• MGC23107
• PKND
• PYCD

Background

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-K Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-K substrate sequence LR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-K will cleave the synthetic substrate LR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CTSK