Human Cathepsin K ELISA Kit (Colorimetric)

ELISA: Human Cathepsin K ELISA Kit (Colorimetric) [NBP2-62755] - Samples were spiked with high concentrations of Human Cathepsin K and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Cathepsin K ELISA Kit (Colorimetric) [NBP2-62755] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Human Cathepsin K ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CTSK in samples. No significant cross-reactivity or interference between Human CTSk and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.91%
• Intra-Assay: CV% < 5.16%
• Spike Recovery: 86-113%
• Sample Volume: 100ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CTSK

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Cathepsin K ELISA Kit (Colorimetric)

• cathepsin K (pycnodysostosis)
• Cathepsin K
• Cathepsin O
• cathepsin O1
• Cathepsin O2
• Cathepsin X
• CTS02
• CTSK
• CTSO
• CTSO1
• CTSO2
• EC 3.4.22
• EC 3.4.22.38
• MGC23107
• PKND
• PYCD

Background

Cathepsin K is encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Cathepsin K ELISA Kit (NBP2-62755)(1)

Publication using NBP2-62755

• Boruk M, Railwah C, Lora A et al. Elevated S100A9 expression in chronic rhinosinusitis coincides with elevated MMP production and proliferation in vitro Sci Rep 2020-10-01 [PMID: 33005006]

FAQs for Cathepsin K ELISA Kit (NBP2-62755). (Showing 1 - 2 of 2 FAQ).

1. Have you used baculovirus to produce these products? NBP2-75332, NBP2-75319, NBP2-62755
   • Baculovirus is not used in the production  of any components of any of these ELISA kits.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CTSK