Cathepsin D Assay Kit (Fluorometric)

Product Discontinued

Cathepsin D Assay Kit (Fluorometric) Summary

• Description: Rapid and convenient fluorometric assay to measure Cathepsin D activity within 1-2 hrs.
  
  Detection method: Fluorescence microscopy, Flow cytometry and Fluorescence plate reader (Ex/Em = 328/460 nm)Applications: Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65302)
• Kit Type: Assay Kit (Fluorometric)
• Gene: CTSD

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Cathepsin D Assay Kit (Fluorometric)

• cathepsin D (lysosomal aspartyl protease)
• Cathepsin D
• CPSD
• CTSD
• EC 3.4.23
• EC 3.4.23.5
• lysosomal aspartyl peptidase
• lysosomal aspartyl protease
• MGC2311
• neuronal 10

Background

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-D Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2) labeled with MCA. Cell lysates or other samples that contain cathepsin-D will cleave the synthetic substrate to release fluorescence, which can then easily be quantified using a fluorometer or fluorescence plate reader at Ex/Em = 328/460 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CTSD