Cathepsin D Antibody (4G2) - BSA Free

Western Blot: Cathepsin D Antibody (4G2) [NBP1-04278] - Western Blot: Cathepsin D antibody (clone 4G2)[NBP04278]-Cell lysates of HepG2, Hep3B, MDA-MB-231 and MCF7 (each 20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system.

Immunohistochemistry-Paraffin: Cathepsin D Antibody (4G2) [NBP1-04278] - Cathepsin D 1:50 on tonsil, pH 9 antigen retrieval. IHC-P image submitted by a verified customer review.

Flow Cytometry: Cathepsin D Antibody (4G2) [NBP1-04278] - Analysis of Cathepsin D in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

Western Blot: Cathepsin D Antibody (4G2) [NBP1-04278] - The recombinant proteins (100ng) were resolved by SDSPAGE, transferred to PVDF membrane and probed with anti human Cathepsin D antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant Human CTSBLane 2. : Recombinant Human CTSDLane 3. : Recombinant Human CTSELane 4. : Recombinant Human CTSFLane 5. : Recombinant Human CTSHLane 6. : Recombinant Human CTSKLane 7. : Recombinant Human CTSLLane 8. : Recombinant Human CTSSLane 9. : Recombinant Human CTSWLane 10. : Recombinant Human CTSZ

Immunohistochemistry-Paraffin: Cathepsin D Antibody (4G2) [NBP1-04278] - human Cathepsin D (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Product Discontinued

Cathepsin D Antibody (4G2) - BSA Free Summary

• Immunogen: Recombinant human Cathepsin D (21-412 aa) purified from E. coli (NP_001900).
• Marker: Lysosomes Marker
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTSD
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: ICC/IF reactivity reported in scientific literature (PMID: 21264228), EM reactivity reported in scientific literature (PMID: 21264221).
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Cathepsin D Antibody (4G2) - BSA Free

• cathepsin D (lysosomal aspartyl protease)
• Cathepsin D
• CPSD
• CTSD
• EC 3.4.23
• EC 3.4.23.5
• lysosomal aspartyl peptidase
• lysosomal aspartyl protease
• MGC2311
• neuronal 10

Background

The aspartic protease cathepsin D is one of the major proteolytic enzymes in lysosomes. The cathepsin D gene locates on chromosome 11p15.5 and spans approximately 11 kb with nine exons. Initially synthesized as an inactive precursor of pro-cathepsin D, the enzyme is subsequently converted to its active forms by proteolytic processing. Breast cancer cells, unlike normal cells, secrete high levels of pro-cathepsin D

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin D Antibody (NBP1-04278)(6)

Publications using NBP1-04278

• Martin T, Juarros M, Cleveland J et al. Assessment of Autophagy Markers Suggests Increased Activity Following LVAD Therapy JACC: Basic to Translational Science 2023-09-01 [PMID: 37791310] (WB, Human)
• Dixit B, Vanhoozer S, Anti N et al. Rapid enrichment of mitochondria from mammalian cell cultures using digitonin MethodsX 2021-08-26 [PMID: 34434723]
• Oldoni F, van Capelleveen JC, Dalila N. Naturally Occurring Variants in LRP1 (Low-Density Lipoprotein Receptor-Related Protein 1) Affect HDL (High-Density Lipoprotein) Metabolism Through ABCA1 (ATP-Binding Cassette A1) and SR-B1 (Scavenger Receptor Class B Type 1) in Humans Arteriosclerosis, Thrombosis, and Vascular Biology 2018-01-01 [PMID: 29853565] (WB, Human)
• Necchi V, Sommi P, Vanoli A et al. Natural history of Helicobacter pylori VacA toxin in human gastric epithelium in vivo: vacuoles and beyond Sci Rep. 2017-11-06 [PMID: 29109534] (Human)
• Kitamura N, Nakamura Y, Miyamoto Y et al. Mieap, a p53-inducible protein, controls mitochondrial quality by repairing or eliminating unhealthy mitochondria PLoS One 2011-01-17 [PMID: 21264228] (ICC/IF, Human)
• Miyamoto Y, Kitamura N, Nakamura Y et al. Possible existence of lysosome-like organella within mitochondria and its role in mitochondrial quality control. PLoS One. 2011-01-01 [PMID: 21264221] (EM, Human)

Review for Cathepsin D Antibody (NBP1-04278) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 01/16/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human Tonsil tissue
• Species: Human
• Lot: I don't have it any more

Comments

• Comments: pH 9 antigen retrieval

FAQs for Cathepsin D Antibody (NBP1-04278). (Showing 1 - 1 of 1 FAQs).

1. I was trying to find out how to make dilutions for this antibody for western blots.
   • For NBP1-04278 Cathepsin D Antibody (4G2), we recommend a 1:1000 dilution but recommend optimization by the end user.

Bioinformatics

• Gene Symbol: CTSD
• Entrez:
  • 1509(Human)
• Uniprot:
  • P07339()