Cathepsin D Antibody (4G2)

Images

 
Western Blot: Cathepsin D Antibody (4G2) [NBP1-04278] - Western Blot: Cathepsin D antibody (clone 4G2)[NBP04278]-Cell lysates of HepG2, Hep3B, MDA-MB-231 and MCF7 (each 20ug) were resolved by SDS-PAGE, transferred to ...read more
Immunohistochemistry-Paraffin: Cathepsin D Antibody (4G2) [NBP1-04278] - Cathepsin D 1:50 on tonsil, pH 9 antigen retrieval. IHC-P image submitted by a verified customer review.
Flow Cytometry: Cathepsin D Antibody (4G2) [NBP1-04278] - Analysis of Cathepsin D in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
Western Blot: Cathepsin D Antibody (4G2) [NBP1-04278] - The recombinant proteins (100ng) were resolved by SDSPAGE, transferred to PVDF membrane and probed with anti human Cathepsin D antibody (1:1000). Proteins were ...read more
Immunohistochemistry-Paraffin: Cathepsin D Antibody (4G2) [NBP1-04278] - human Cathepsin D (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-P
Clone
4G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Cathepsin D Antibody (4G2) Summary

Immunogen
Recombinant human Cathepsin D (21-412 aa) purified from E. coli (NP_001900).
Marker
Lysosomes Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CTSD
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Electron Microscopy
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
ICC/IF reactivity reported in scientific literature (PMID: 21264228), EM reactivity reported in scientific literature (PMID: 21264221).
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-04278 in the following applications:

Publications
Read Publications using
NBP1-04278 in the following applications:

  • EM
    1 publication
  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Cathepsin D Antibody (4G2)

  • cathepsin D (lysosomal aspartyl protease)
  • Cathepsin D
  • CPSD
  • CTSD
  • EC 3.4.23
  • EC 3.4.23.5
  • lysosomal aspartyl peptidase
  • lysosomal aspartyl protease
  • MGC2311
  • neuronal 10

Background

The aspartic protease cathepsin D is one of the major proteolytic enzymes in lysosomes. The cathepsin D gene locates on chromosome 11p15.5 and spans approximately 11 kb with nine exons. Initially synthesized as an inactive precursor of pro-cathepsin D, the enzyme is subsequently converted to its active forms by proteolytic processing. Breast cancer cells, unlike normal cells, secrete high levels of pro-cathepsin D

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin D Antibody (NBP1-04278)(4)

Review for Cathepsin D Antibody (NBP1-04278) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-04278:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Cathepsin D NBP1-04278
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reviewed by:
Anonymous
IHC-P Human 01/16/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman Tonsil tissue
SpeciesHuman
LotI don't have it any more

Comments

CommentspH 9 antigen retrieval

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cathepsin D Antibody (NBP1-04278) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-04278AF350
Alexa Fluor 405 NBP1-04278AF405
Alexa Fluor 488 NBP1-04278AF488
Alexa Fluor 532 NBP1-04278AF532
Alexa Fluor 594 NBP1-04278AF594
Alexa Fluor 647 NBP1-04278AF647
Alexa Fluor 700 NBP1-04278AF700
Alexa Fluor 750 NBP1-04278AF750
Biotin NBP1-04278B
DyLight 350 NBP1-04278UV
DyLight 405 NBP1-04278V
DyLight 488 NBP1-04278G
DyLight 550 NBP1-04278R
DyLight 594 NBP1-04278DL594
DyLight 650 NBP1-04278C
DyLight 680 NBP1-04278FR
DyLight 755 NBP1-04278IR
FITC NBP1-04278F
HRP NBP1-04278H
Janelia Fluor 549 NBP1-04278JF549
Janelia Fluor 646 NBP1-04278JF646
PerCP NBP1-04278PCP

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Contact Information

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
01/16/2020
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CTSD
Entrez
Uniprot
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