Western Blot: Cathepsin D Antibody (4G2) [NBP1-04278] - Western Blot: Cathepsin D antibody (clone 4G2)[NBP04278]-Cell lysates of HepG2, Hep3B, MDA-MB-231 and MCF7 (each 20ug) were resolved by SDS-PAGE, transferred to ...read more
Immunohistochemistry-Paraffin: Cathepsin D Antibody (4G2) [NBP1-04278] - Cathepsin D 1:50 on tonsil, pH 9 antigen retrieval. IHC-P image submitted by a verified customer review.
Flow Cytometry: Cathepsin D Antibody (4G2) [NBP1-04278] - Analysis of Cathepsin D in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
Western Blot: Cathepsin D Antibody (4G2) [NBP1-04278] - The recombinant proteins (100ng) were resolved by SDSPAGE, transferred to PVDF membrane and probed with anti human Cathepsin D antibody (1:1000). Proteins were ...read more
Immunohistochemistry-Paraffin: Cathepsin D Antibody (4G2) [NBP1-04278] - human Cathepsin D (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using ...read more
ICC/IF reactivity reported in scientific literature (PMID: 21264228), EM reactivity reported in scientific literature (PMID: 21264221).
44 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-04278 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4), 10% Glycerol
0.02% Sodium Azide
Protein G purified
Alternate Names for Cathepsin D Antibody (4G2)
cathepsin D (lysosomal aspartyl protease)
lysosomal aspartyl peptidase
lysosomal aspartyl protease
The aspartic protease cathepsin D is one of the major proteolytic enzymes in lysosomes. The cathepsin D gene locates on chromosome 11p15.5 and spans approximately 11 kb with nine exons. Initially synthesized as an inactive precursor of pro-cathepsin D, the enzyme is subsequently converted to its active forms by proteolytic processing. Breast cancer cells, unlike normal cells, secrete high levels of pro-cathepsin D
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.