Cathepsin B Antibody [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cathepsin B knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin B at approximately 34 kDa (as indicated) using 2.5 µg/mL of Goat ...read more
Cathepsin B was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) at 10 µg/mL overnight at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Cathepsin B Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin B
Arg18-Ile339
Accession # P07858
Specificity
Detects human Cathepsin B in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse (rm) Cathepsin B is observed and less than 5% cross-reactivity with recombinant human (rh) Cathepsin C, rmCathepsin H, and rhCathepsin L is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSB
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 2.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Knockout Validated
Control
Cathepsin B Knockout HeLa Cell Lysate
Publications
Read Publications using
AF953 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin B Antibody [Unconjugated]

  • APP secretase
  • APPS
  • Cathepsin B
  • Cathepsin B1
  • CPSBamyloid precursor protein secretase
  • CTSB
  • cysteine protease
  • EC 3.4.22
  • EC 3.4.22.1

Background

Cathepsin B is the first described member of the family of lysosomal cysteine proteases (1). Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins, including pro and active caspases, prorenin, and secretory leucoprotease inhibitor (SLPI) (2-4). Therefore, Cathepsin B may play a role in activation and inactivation of caspases, activation of renin and inactivation of SLPI, the key steps in apoptosis, angiotensin production, and progression of emphysema, respectively. Because of its increased levels and redistribution of the enzyme in human and animal tumors, Cathepsin B may also have role in invasion and metastasis (5).

In addition to lysosome, Cathepsin B can be secreted or associated with plasma membrane, cytoplasm, and nucleus. It is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme (1).

  1. Mort, J.S. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds): Academic Press, San Diego, p. 1079.
  2. Vancompernolle, K. et al. (1998) FEBS Lett. 438:150.
  3. Jutras, I. and T.L. Reudelhuber (1999) FEBS Lett. 443:48.
  4. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
  5. Bergquin, I.M. and B.F. Sloane (1996) Adv. Exp. Med. Biol. 389:281.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin B Antibody (AF953)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 4 applications: ICC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ICC
(1)
IHC-Fr
(1)
IHC-P
(3)
Western Blot
(10)
All Applications
Filter By Species
Human
(12)
Mouse
(1)
Rat
(1)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF953 Applications Species
Y Xu, J Ren, X He, H Chen, T Wei, W Feng YWHA/14-3-3 proteins recognize phosphorylated TFEB by a noncanonical mode for controlling TFEB cytoplasmic localization Autophagy, 2019;0(0):1-14. 2019 [PMID: 30653408] (Western Blot, Human) Western Blot Human
AP Tran, S Sundar, M Yu, BT Lang, J Silver Modulation of Receptor Protein Tyrosine Phosphatase Sigma Increases Chondroitin Sulfate Proteoglycan Degradation through Cathepsin B Secretion to Enhance Axon Outgrowth J. Neurosci., 2018;38(23):5399-5414. 2018 [PMID: 29760175] (Western Blot, Rat) Western Blot Rat
CW Lee, JN Stankowski, J Chew, CN Cook, YW Lam, S Almeida, Y Carlomagno, KF Lau, M Prudencio, FB Gao, M Bogyo, DW Dickson, L Petrucelli The lysosomal protein cathepsin L is a progranulin protease Mol Neurodegener, 2017;12(1):55. 2017 [PMID: 28743268] (Western Blot, Human) Western Blot Human
The Unusual Resistance of Avian Defensin AvBD7 to Proteolytic Enzymes Preserves Its Antibacterial Activity PLoS ONE, 2016;11(8):e0161573. 2016 [PMID: 27561012] (Western Blot, Chicken) Western Blot Chicken
Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors Sci Rep, 2016;6(0):29256. 2016 [PMID: 27387133] (Western Blot, Human) Western Blot Human
Smith R, Solberg R, Jacobsen L, Voreland A, Rustan A, Thoresen G, Johansen H Simvastatin inhibits glucose metabolism and legumain activity in human myotubes. PLoS ONE, 2014;9(1):e85721. 2014 [PMID: 24416446] (Western Blot, Human) Western Blot Human
Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, Lam P Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis. Stem Cells, 2013;31(1):146-55. 2013 [PMID: 23034897] (IHC-Fr, Human) IHC-Fr Human
Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis. Cell Res., 2012;22(9):1356-73. 2012 [PMID: 22688893] (Western Blot, Human) Western Blot Human
Noda S, Asano Y, Akamata K, Aozasa N, Taniguchi T, Takahashi T, Ichimura Y, Toyama T, Sumida H, Yanaba K, Tada Y, Sugaya M, Kadono T, Sato S A possible contribution of altered cathepsin B expression to the development of skin sclerosis and vasculopathy in systemic sclerosis. PLoS ONE, 2012;7(2):e32272. 2012 [PMID: 22384200] (IHC-P, Human) IHC-P Human
Shree T, Olson OC, Elie BT Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer. Genes Dev., 2011;25(23):2465-79. 2011 [PMID: 22156207] (IHC-P, Human) IHC-P Human
Show All 15 Publications.

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF953

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Cathepsin B - a lysosomal protease with potential of an important drug target in neurological diseases and cancer
Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.

Caspase 11: A novel non-canonical inflammasomes
Cell death via apoptosis is a key cellular function triggered by the cell death receptor family and their ligands. This regulated process then transmits downstream signals through adaptor molecules ending with the caspase cysteine proteases. Caspas...  Read full blog post.

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Bioinformatics

Gene Symbol CTSB
Uniprot