Catenin alpha 1 [p Tyr148] Antibody

Western Blot: Catenin alpha 1 [p Tyr148] Antibody [NBP3-23196] - Western blot analysis of rat PC12 cells treated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti- -Catenin monoclonal (lanes 1 & 2), anti-Catenin alpha 1 (Tyr-148) phospho-specific (lanes 3 & 4), or anti-Catenin alpha 1 (a.a. 143-153) (lanes 5 & 6).

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Catenin alpha 1 [p Tyr148] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-Catenin alpha 1 (Tyr-148) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 148 in human Catenin alpha 1. This peptide sequence is highly conserved in rat and mouse Catenin alpha 1, but is not conserved in Catenin alpha 2 or Catenin alpha 3. [UniProt# P35221]
• Modification: p Tyr148
• Specificity: This antibody was cross-adsorbed to unrelated phospho-tyrosine peptide before affinity purification using phospho-Catenin alpha 1 (Tyr-148) peptide (without carrier). The antibody detects a 102 kDa protein corresponding to the molecular mass of a1-Catenin on SDS-PAGE immunoblots of rat PC12 cells treated with pervanadate.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTNNA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 102 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Catenin alpha 1 [p Tyr148] Antibody

• Alpha E-catenin
• alpha-catenin
• alpha-E-catenin
• alphaE-catenin
• cadherin-associated protein
• CAP102
• catenin (cadherin-associated protein), alpha 1 (102kD)
• catenin (cadherin-associated protein), alpha 1, 102kDa
• catenin alpha-1
• FLJ36832
• FLJ52416
• Renal carcinoma antigen NY-REN-13

Background

Cadherin and catenin compose cell adhesion complex and are indispensable for tight cell-cell adhesion. Dysfunction of this adhesion complex causes dissociation of cancer cells from primary tumor nodules, thus possibly contributing to cancer invasion and metastasis (1). At least three proteins (alpha, beta, and gamma catenin) comprise the cytoplasmic domain of the cadherin cell-cell adhesion complex. Data, with the reported structure of other catenin genes, suggest that vinculin and alpha-catenin generate a superfamily of proteins mediating membrane-cytoskeletal associations (2). Presenilin-1 (PS1) overexpression in human kidney cells enhances cell-cell adhesion and data show that PS1 incorporates into the cadherin/catenin adhesion system and regulates cell-cell adhesion. PS1 concentrates at intercellular contacts in epithelial tissue; in brain, it forms complexes with both E- and N-cadherin and concentrates at synaptic adhesions (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTNNA1