Catalase Antibody

Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Product Discontinued

Catalase Antibody Summary

• Immunogen: The catalase enzyme from bovine liver was used as the immunogen.
• Marker: Peroxisome Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.1-0.5 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 ml PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Catalase Antibody

• Cas1
• CAT
• Catalase
• Cs-1
• EC 1.11.1.6
• MGC138422
• MGC138424

Background

Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1.5A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erthrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflamation) (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Catalase Antibody (NBP2-24916)(10)

Publications using NBP2-24916

• Yang TY, Wu YL, Yu MH et al. Mulberry Leaf and Neochlorogenic Acid Alleviates Glucolipotoxicity-Induced Oxidative Stress and Inhibits Proliferation/Migration via Downregulating Ras and FAK Signaling Pathway in Vascular Smooth Muscle Cell Nutrients 2022-07-22 [PMID: 35893859] (WB, Rat)
• Choi S, Kim J, Li H Et al. Anti-inflammatory and anti-fibrotic effects of modafinil in nonalcoholic liver disease Biomedicine & Pharmacotherapy 2021-12-01 [PMID: 34794237] (WB, Mouse, Human)
• Rahman MS, Rahman MS Elevated seasonal temperature disrupts prooxidant-antioxidant homeostasis and promotes cellular apoptosis in the American oyster, Crassostrea virginica, in the Gulf of Mexico: a field study Cell stress & chaperones 2021-09-15 [PMID: 34524641]
• Pek E, Canbey Goret C, Hacivelioglu S et al. The immunohistochemical and histologic effects of contrast medium on uterus, fallopian tubes and ovaries, given during hysterosalpingography: rat study Journal of the Turkish German Gynecological Association 2020-12-04 [PMID: 32500677] (IHC-P, Rat)
• Manoharan B, Bobby Z, Dorairajan G Increased placental expressions of nuclear factor erythroid 2-related factor 2 and antioxidant enzymes in gestational diabetes: Protective mechanisms against the placental oxidative stress? Eur. J. Obstet. Gynecol. Reprod. Biol. 2019-05-15 [PMID: 31121342] (IF/IHC, WB, Human)
• Mishra P, Paital B, Jena S et al. Possible activation of NRF2 by Vitamin E/Curcumin against altered thyroid hormone induced oxidative stress via NF kappa B/AKT/mTOR/KEAP1 signalling in rat heart Sci Rep 2019-05-15 [PMID: 31092832] (WB, Rat)
• Roma A, Ovadje P, Steckle M et al. Selective Induction of Apoptosis by Azadarichta indica Leaf Extract by Targeting Oxidative Vulnerabilities in Human Cancer Cells. J Pharm Pharm Sci. 2015-11-01 [PMID: 26626256] (WB, Human)
  
  Details:
  SOD1/Cu-Zn SOD antibody was used for WB analysis of lysates from Jurkat /E6-1 cells that were treated or not with aqueous or ethanolic Azadarichta indica/neem leaf extracts (Figure 7).
• Yang SP, Yang XZ, Cao GP. Acetyl-l-carnitine prevents homocysteine-induced suppression of Nrf2/Keap1 mediated antioxidation in human lens epithelial cells. Mol Med Rep 2015-07-01 [PMID: 25776802]
• Sagar S, Kumar P, Behera RR, Pal A. Effects of CEES and LPS synergistically stimulate oxidative stress inactivates OGG1 signaling in macrophage cells. J. Hazard. Mater. 2014-06-06 [PMID: 24976129] (WB, Mouse)
  
  Details:
  Fig 6: RAW264.7
• Kumar P, Rao GN, Pal BB, Pal A. Hyperglycemia-induced oxidative stress induces apoptosis by inhibiting PI3-kinase/Akt and ERK1/2 MAPK mediated signaling pathway causing downregulation of 8-oxoG-DNA glycosylase levels in glial cells. Int. J. Biochem. Cell Biol. 2014-06-04 [PMID: 24907397] (WB, Rat)
  
  Details:
  Fig 2B: primary astroglial cells. Catalase was detected below the 72 kDa marker.

Review for Catalase Antibody (NBP2-24916) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/01/2017

Summary

• Application: Western Blot
• Sample Tested: Placental tissue
• Species: Human
• Lot: 03254363C3B

Bioinformatics

• Gene Symbol: CAT
• Uniprot:
  • P04040()