Catalase Antibody (724810) [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Catalase Monoclonal Antibody (Catalog # MAB3398) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Catalase at approximately 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for Catalase at approximately 61 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Catalase Monoclonal Antibody (Catalog # MAB3398) . This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Catalase was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Catalase Monoclonal Antibody (Catalog # MAB3398) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to peroxisomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Catalase knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Catalase Monoclonal Antibody (Catalog # MAB3398) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Catalase at approximately 64 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF, KO
• Clone: 724810
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Catalase Antibody (724810) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Catalase
  Met1-Leu527
  Accession # P04040
• Specificity: Detects human Catalase in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Catalase or recombinant human Serpin C1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAT
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Knockout Validated
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Catalase Antibody (724810) [Unconjugated]

• Cas1
• CAT
• Catalase
• Cs-1
• EC 1.11.1.6
• MGC138422
• MGC138424

Background

Cells have evolved complex mechanisms to maintain redox balance and defend against oxidative stress. Catalase is a tetrameric enzyme comprised of four 60 kDa subunits. Catalase is typically localized in the peroxisome where it functions as an antioxidant, protecting cells from damage due to oxidative stress. Catalase converts reactive oxygen species, such as H₂O₂, into water and O₂. Human Catalase shares 89% homology to mouse and rat Catalase. The cells redox environment can serve as an important signaling switch or trigger to initiate a number of cellular processes, including gene expression, differentiation, proliferation and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Catalase Antibody (MAB3398)(3)

Publications using MAB3398

• Wilhelm LP, Zapata-MuNoz J, Villarejo-Zori B et al. BNIP3L/NIX regulates both mitophagy and pexophagy The EMBO journal 10/10/2022 [PMID: 36215693]
• KS Specht, S Kant, AK Addington, RP McMillan, MW Hulver, H Learnard, M Campbell, SR Donnelly, AD Caliz, Y Pei, MM Reif, JM Bond, A DeMarco, B Craige, JF Keaney, SM Craige Nox4 mediates skeletal muscle metabolic responses to exercise Molecular Metabolism, 2021-01-02;45(0):101160. 2021-01-02 [PMID: 33400973] (Western Blot, Mouse)
• Vuong Cat Khanh, Toshiharu Yamashita, Kinuko Ohneda, Chiho Tokunaga, Hideyuki Kato, Motoo Osaka et al. Rejuvenation of mesenchymal stem cells by extracellular vesicles inhibits the elevation of reactive oxygen species Scientific Reports 2020-10-14 [PMID: 33057147]

Bioinformatics

• Gene Symbol: CAT
• Uniprot:
  • P04040()