Recombinant Human Caspase-9 Protein

Product Discontinued

Recombinant Human Caspase-9 Protein Summary

• Description: Recombinant protein for Human Caspase-9
  
  Source:E. coli.
  Amino Acid Sequence:(P55211)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab52203)
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CASP9
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Each Definition: One unit of the recombinant caspase-9 is the enzyme activity that cleaves 1 nmol of the caspase substrate LEHD-pNA (pNA: pnitroanaline) per hour at 37C in a reaction solution containing 50 mM Hepes pH 7.2 50 mM NaCl 0.1% Chaps 10 mM EDTA 5% Glycerol and 10 mM DTT.
  Specific activity: > 400 units/mg

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Semi Dry powder
• Concentration: Lyoph
• Purity: >90%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute to 1 unit per l in PBS containing 15% glycerol.

Alternate Names for Recombinant Human Caspase-9 Protein

• APAF-3APAF3
• apoptotic protease activating factor 3
• Apoptotic protease Mch-6
• Apoptotic protease-activating factor 3
• CASP9
• CASP-9
• caspase 9, apoptosis-related cysteine peptidase
• Caspase9
• Caspase-9
• EC 3.4.22.62
• ICE-LAP6CASPASE-9c
• ICE-like apoptotic protease 6
• MCH6apoptosis-related cysteine protease

Background

Caspase-9 is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-9 also exists in cells as an inactive proenzyme. During the initiation of apoptosis procaspase-9 is processed at aspartate residues to form active caspase-9. As one of the initiator caspases, active caspase-9 functions to trigger activation of downstream effector caspases, leading to disassembly of cell structures. The recombinant active human caspase-9 was expressed in E. coli. The active caspase-9 is routinely tested at for its ability to enzymatically cleave these two substrates Ac-LEHD-pNA (Cat. #1076-200) or Ac-LEHD-AFC (1075-200).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for Caspase-9 Recombinant Protein (NBP1-99606). (Showing 1 - 1 of 1 FAQ).

1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
   • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Bioinformatics

• Gene Symbol: CASP9