Caspase-9 Assay Kit (Fluorometric)

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Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications Flow
Conjugate
Rhodamine
Suitable Sample Type
Live cells

Order Details

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Catalog# & Conjugate Size Price

Caspase-9 Assay Kit (Fluorometric) Summary

Description
Activation of caspases plays a central role in apoptosis. This kit provides a convenient means for detecting activated caspase-9 in living cells. The assay utilizes a caspase-9 inhibitor LEHD-FMK conjugated to sulfo-rhodamine (Red-LEHD-FMK) as the fluorescent marker. Red-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells..
  • Detection method: Fluorescence microscopy, Flow cytometry or Fluorescence plate reader (Ex/Em = 540/570 nm).
  • Applications: Detection of activated caspase-9 in living cells.
Specificity
For the active/cleaved form of Caspase-9.
Kit Type
Assay Kit (Fluorometric)
Gene
CASP9

Applications/Dilutions

Publications
Read Publication using
NBP2-54872 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. Red-LEHD-FMK
  2. Wash Buffer
  3. Z-VAD-FMK

Alternate Names for Caspase-9 Assay Kit (Fluorometric)

  • APAF-3APAF3
  • apoptotic protease activating factor 3
  • Apoptotic protease Mch-6
  • Apoptotic protease-activating factor 3
  • CASP9
  • CASP-9
  • caspase 9, apoptosis-related cysteine peptidase
  • Caspase9
  • Caspase-9
  • EC 3.4.22.62
  • ICE-LAP6CASPASE-9c
  • ICE-like apoptotic protease 6
  • MCH6apoptosis-related cysteine protease

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Publications for Caspase-9 Kit (NBP2-54872)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.


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ELISA
(1)
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Human
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-9 Kit (NBP2-54872). (Showing 1 - 1 of 1 FAQ).

  1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
    • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Other Available Formats

Rhodamine NBP2-54872
Rhodamine NBP2-54872

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Blogs on Caspase-9.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Bioinformatics

Gene Symbol CASP9