Caspase-9 Antibody

Western Blot: Caspase-9 Antibody - Unpurified [NB100-56120] - Caspase-9 Antibody [NB100-56120] - Recombinant human Caspase-9 probed with this antibody at 1:1000.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Caspase-9 Antibody Summary

• Immunogen: A synthetic peptide corresponding to amino acids 113-130 (PEIRKPEVLRPETPRPVD) of human caspase-9 was used as immunogen; GenBank no. gi|55960247|emb|CAI12974.1|.
• Specificity: This polyclonal antisera recognizes the proform of caspase-9 (~50 kDa), and the large (~35 kDa) subunit (with pro-domain sequences) of active/cleaved Caspase-9. It does not recognize the small (~15 kDa) subunit of active/cleaved Caspase-9. Intermediate caspase-9 cleavage forms may also be seen at ~21 kDa.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP9
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200

Reactivity Notes

Mouse, Canine, Rat reactivity reported in scientific literature (PMID: 15380478).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for Caspase-9 Antibody

• APAF-3APAF3
• apoptotic protease activating factor 3
• Apoptotic protease Mch-6
• Apoptotic protease-activating factor 3
• CASP9
• CASP-9
• caspase 9, apoptosis-related cysteine peptidase
• Caspase9
• Caspase-9
• EC 3.4.22.62
• ICE-LAP6CASPASE-9c
• ICE-like apoptotic protease 6
• MCH6apoptosis-related cysteine protease

Background

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Caspase-9 polyclonal antisera recognizes the proform of caspase-9 (approx. 50 kDa), and the large (approx. 35 kDa) subunit (with pro-domain sequences) of active/cleaved Caspase-9. It does not recognize the small (approx. 15 kDa) subunit of active/cleaved Caspase-9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-9 Antibody (NB100-56120)(2)

Publications using NB100-56120

• Krajewska M, Rosenthal RE, Mikolajczyk J et al. Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation. Exp Neurol. 2004 Oct [PMID: 15380478] (WB, IHC-P, Mouse, Canine, Rat)
  
  Details:
  Antibodies cited: 1. Caspase-9 (Active/Cleaved), IMG-5705: WB: Fig 1A (recombinant human caspase-9), Fig 4A (recombinant human caspase-9, rat brain), Fig 4B-F (isolated mitochondria from rat liver or heart). Fig 5 (PC12 cells) IHC (P): Fig 2d-k (rat kidne
• Krajewski S, Krajewska M, Ellerby LM et al. Release of caspase-9 from mitochondria during neuronal apoptosis and cerebral ischemia. Proc Natl Acad Sci U S A. 1999 May 11 [PMID: 10318956]

FAQs for Caspase-9 Antibody (NB100-56120). (Showing 1 - 1 of 1 FAQs).

1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
   • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Bioinformatics

• Gene Symbol: CASP9
• Entrez:
  • 842(Human)