Caspase-8 Antibody - (Pro and Active)

Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP1-05123] - Formalin-fixed, paraffin-embedded sections from a brain tumor tissue array stained for Caspase-8 expression using NBP1-05123 at 1:2000. A. Anaplastic glioma cores from two different patients, positive (left) and negative (right) staining for caspase-8. B. Higher magnification from the caspase-8 positive (A, left) core.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-Fr, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Caspase-8 Antibody - (Pro and Active) Summary

• Immunogen: Synthetic peptide corresponding to the C-terminus of human Caspase-8 (PQPTFTLRKKLVFPSD). This sequence is common to various Caspase-8 forms including: 1. Caspase 8a (aa 481-496 GenBank no. NP_001219.2) 2. Caspase 8b (aa 464-479, GenBank no. NP_203519.1) 3. Caspase 8c (aa 449-464, GenBank no. NP_203520.1) 4. Caspase 8L (aa 523-538, GenBank no. gbAAL87628.1) 5. Truncated caspase 8 (as 251-266, GenBank no. gi3928274)
• Specificity: Caspase-8
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP8
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
• Control:

  Caspase-8 Knockout HeLa Cell Lysate
  Caspase-8 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Caspase-8 Antibody - (Pro and Active)

• AIS
• androgen receptor
• CASP8
• Caspase8
• Caspase-8
• DHTRTFM
• Dihydrotestosterone receptorHYSP1
• HUMARA
• Mch5
• NR3C4KD
• Nuclear receptor subfamily 3 group C member 4
• SMAX1SBMA
• spinal and bulbar muscular atrophy

Background

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are constitutively expressed in almost all cell types as inactive proenzymes (zymogens: enzyme precursors which require a biochemical change to become active enzymes) that are processed and activated in response to a variety of pro-apoptotic or inflammatory stimuli. The procaspases (32-56 kDa) contain four domains: an N-terminal prodomain (2-25 kDa), a large subunit (p20: 17-21 kDa), a small subunit (p10: 10-13 kDa) and a short linker region between the large and small subunits. Caspase activation involves proteolytic processing of the proenzyme at specific aspartate residues between the domains. This results in removal of the prodomain as well as the linker region and formation of a heterodimer containing one large and one small subunit (p20-p10). The active caspase is a tetramer composed of two heterodimers (p202-p102). Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. Multiple isoforms of caspase 8 have been identified, including caspase 8a [496 amino acids (aa), GenBank no. NP_001219.2], caspase 8b (479 aa, Gen Bank no. NP_203519.1), caspase 8c (464 aa, GenBank no. NP_203520.1), and caspase 8L (538 aa, GenBank no. gbAAL87628.1). Truncated caspase-8 forms have also been described. Users are encoraged to go to the NCBI ACE View Data Base for more information. http://www.ncbi.nih.gov/IEB/Research/Acembly/ Caspase-8 may be observed on Western blots at varying apparent molecular weights depending on isoform, truncation, or cleavage forms present.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-8 Antibody (NBP1-05123)(4)

Publications using NBP1-05123

• Biernacki M, Luczaj W, Jarocka-Karpowicz I et al. The Effect of Long-Term Administration of Fatty Acid Amide Hydrolase Inhibitor URB597 on Oxidative Metabolism in the Heart of Rats with Primary and Secondary Hypertension. Molecules Sep 14 2018 [PMID: 30223427] (WB, Rat)
• Tian F, Clift MJ, Casey A et al. Investigating the role of shape on the biological impact of gold nanoparticles in vitro. Nanomedicine (Lond) 2015 Sep 01 [PMID: 26377045]
• Liu Q, Zhao X, Frissora F et al. FTY720 demonstrates promising preclinical activity for chronic lymphocytic leukemia and lymphoblastic leukemia/lymphoma. Blood. 2008 Jan 1 [PMID: 17761520]
• Svingen PA, Karp JE, Krajewski S et al. Evaluation of Apaf-1 and procaspases-2, -3, -7, -8, and -9 as potential prognostic markers in acute leukemia. Blood. 2000 Dec 1 [PMID: 11090079]

FAQs for Caspase-8 Antibody (NBP1-05123). (Showing 1 - 1 of 1 FAQs).

1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
   • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Bioinformatics

• Gene Symbol: CASP8
• Entrez:
  • 841(Human)
  • 12370(Mouse)
  • 64044(Rat)
• Uniprot:
  • NP_001073595(Mouse)