Caspase-8 Antibody


Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP1-05123] - Formalin-fixed, paraffin-embedded sections from a brain tumor tissue array stained for Caspase-8 expression using NBP1-05123 at 1:2000. A. Anaplastic more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Caspase-8 Antibody Summary

Synthetic peptide corresponding to the C-terminus of human Caspase-8 (PQPTFTLRKKLVFPSD). This sequence is common to various Caspase-8 forms including: 1. Caspase 8a (aa 481-496 GenBank no. NP_001219.2) 2. Caspase 8b (aa 464-479, GenBank no. NP_203519.1) 3. Caspase 8c (aa 449-464, GenBank no. NP_203520.1) 4. Caspase 8L (aa 523-538, GenBank no. gbAAL87628.1) 5. Truncated caspase 8 (as 251-266, GenBank no. gi3928274)
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Whole antisera
0.05% Sodium Azide


  • Western Blot 1:1000-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation
Application Notes
NBP1-05123 has been successfully tested in immunoprecipitation, immunohistochemistry-Paraffin, immunohistochemistry-Frozen and Western blot applications.
Read Publications using NBP1-05123.

Reactivity Notes

NBP1-05123 has cross-reactivity with Gerbil, Human, Mouse, Rat Caspase-8.

Alternate Names for Caspase-8 Antibody

  • AIS
  • androgen receptor
  • CASP8
  • Caspase8
  • Dihydrotestosterone receptorHYSP1
  • Mch5
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • spinal and bulbar muscular atrophy


Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are constitutively expressed in almost all cell types as inactive proenzymes (zymogens: enzyme precursors which require a biochemical change to become active enzymes) that are processed and activated in response to a variety of pro-apoptotic or inflammatory stimuli. The procaspases (32-56 kDa) contain four domains: an N-terminal prodomain (2-25 kDa), a large subunit (p20: 17-21 kDa), a small subunit (p10: 10-13 kDa) and a short linker region between the large and small subunits. Caspase activation involves proteolytic processing of the proenzyme at specific aspartate residues between the domains. This results in removal of the prodomain as well as the linker region and formation of a heterodimer containing one large and one small subunit (p20-p10). The active caspase is a tetramer composed of two heterodimers (p202-p102). Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. Multiple isoforms of caspase 8 have been identified, including caspase 8a [496 amino acids (aa), GenBank no. NP_001219.2], caspase 8b (479 aa, Gen Bank no. NP_203519.1), caspase 8c (464 aa, GenBank no. NP_203520.1), and caspase 8L (538 aa, GenBank no. gbAAL87628.1). Truncated caspase-8 forms have also been described. Users are encoraged to go to the NCBI ACE View Data Base for more information. Caspase-8 may be observed on Western blots at varying apparent molecular weights depending on isoform, truncation, or cleavage forms present.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-8 Antibody (NBP1-05123)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Caspase-8 Antibody (NBP1-05123). (Showing 1 - 1 of 1 FAQs).

  1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
    • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Secondary Antibodies


Isotype Controls

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Bioinformatics Tool for Caspase-8 Antibody (NBP1-05123)

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Research Areas for Caspase-8 Antibody (NBP1-05123)

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Blogs on Caspase-8.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

VPS41 - An important regulator of lysosomal trafficking
Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab...  Read full blog post.

Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology
Caspases are a family of cysteine-aspartic acid proteases that are responsible for the initiation and execution of apoptosis. Caspase-8 is a 55 kDa protein expressed as an inactive procaspase that resides in the cytosol. Activation of Caspase-8 req...  Read full blog post.

Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.

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Product PDFs


Gene Symbol CASP8

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