Caspase-4 Antibody

Western Blot: Caspase-4 Antibody [NB300-901] - Staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Bv, Ca, Fe
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Caspase-4 Antibody Summary

• Description: Novus Biologicals Goat Caspase-4 Antibody (NB300-901) is a polyclonal antibody validated for use in WB and ELISA. Anti-Caspase-4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CKERAEEIYPIKERN corresponding to internal region according to NP_001216.1, NP_150649.1.
• Specificity: This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP_150649.1).
• Predicted Species: Mouse (100%), Feline (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CASP4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in human heart lysates (calculated MW of 43.3 kDa band according to NP_001216.1).
• Control:

  Caspase-4 Overexpression Lysate
  Jurkat Staurosporine Treated / Untreated Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Caspase-4 Antibody

• apoptotic cysteine protease Mih1/TX
• CASP4
• CASP-4
• caspase 4, apoptosis-related cysteine peptidase
• caspase 4, apoptosis-related cysteine protease
• Caspase4
• Caspase-4
• EC 3.4.22
• EC 3.4.22.57
• ICE(rel)II
• ICE(rel)-II
• ICEREL-II
• ICH2
• ICH-2
• Mih1/TX
• Protease ICH-2
• Protease TX
• TX

Background

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-4 Antibody (NB300-901)(1)

Publication using NB300-901

• Munday NA, Vaillancourt JP, Ali A et al. Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases. J Biol Chem 1995-06-30 [PMID: 7797592]

Bioinformatics

• Gene Symbol: CASP4
• Entrez:
  • 837(Human)
• Uniprot:
  • NP_001216(Human)