This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Caspase-3 Assay Kit
apoptosis-related cysteine protease
caspase 3, apoptosis-related cysteine peptidase
CPP32SREBP cleavage activity 1
Cysteine protease CPP32
PARP cleavage protease
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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